Structural basis for specific recognition of multiple mRNA targets by a PUF regulatory protein

Yeming Wang; Laura Opperman; Marvin Wickens; Traci M. Tanaka Hall

首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Structural basis for specific recognition of multiple mRNA targets by a PUF regulatory protein

【24h】

Structural basis for specific recognition of multiple mRNA targets by a PUF regulatory protein

机译：PUF调节蛋白特异性识别多个mRNA靶标的结构基础

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Caenorhabditis elegans fem-3 binding factor (FBF) is a founding member of the PUMILIO/FBF (PUF) family of mRNA regulatory proteins. It regulates multiple mRNAs critical for stem cell maintenance and germline development. Here, we report crystal structures of FBF in complex with 6 different 9-nt RNA sequences, including elements from 4 natural mRNAs. These structures reveal that FBF binds to conserved bases at positions 1-3 and 7-8. The key specificity determinant of FBF vs. other PUF proteins lies in positions 4-6. In FBF/RNA complexes, these bases stack directly with one another and turn away from the RNA-binding surface. A short region of FBF is sufficient to impart its unique specificity and lies directly opposite the flipped bases. We suggest that this region imposes a flattened curvature on the protein; hence, the requirement for the additional nucleotide. The principles of FBF/RNA recognition suggest a general mechanism by which PUF proteins recognize distinct families of RNAs yet exploit very nearly identical atomic contacts in doing so.

机译：秀丽隐杆线虫fem-3结合因子（FBF）是mRNA调控蛋白PUMILIO / FBF（PUF）家族的创始成员。它调节对干细胞维持和种系发育至关重要的多种mRNA。在这里，我们报告与6个不同的9-nt RNA序列，包括来自4个天然mRNA的元素复杂的FBF的晶体结构。这些结构揭示了FBF在位置1-3和7-8处与保守碱基结合。 FBF与其他PUF蛋白的关键特异性决定因素位于位置4-6。在FBF / RNA复合物中，这些碱基彼此直接堆叠，并远离RNA结合表面。 FBF的短区域足以赋予其独特的特异性，并直接位于翻转碱基的对面。我们建议该区域在蛋白质上施加平坦的曲率。因此，需要额外的核苷酸。 FBF / RNA识别的原理提出了一种通用机制，通过这种机制，PUF蛋白可以识别不同的RNA家族，但可以利用它们几乎完全相同的原子接触。

著录项

来源
《Proceedings of the National Academy of Sciences of the United States of America》 |2009年第48期|20186-20191|共6页
作者
Yeming Wang; Laura Opperman; Marvin Wickens; Traci M. Tanaka Hall;
展开▼
作者单位

Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709;

Department of Biochemistry, University of Wisconsin, Madison, WI 53706;

Department of Biochemistry, University of Wisconsin, Madison, WI 53706;

Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709;

展开▼
收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类
关键词
crystal structure; RNA; translational regulation; fem-3 binding factor; base flipping;

机译：晶体结构RNA;翻译规则;fem-3结合因子;基础翻转;

相似文献

外文文献
中文文献
专利

1. "Multiple partial recognitions in dynamic equilibrium" in the binding sites of proteins form the molecular basis of promiscuous recognition of structurally diverse ligands [J] . Daisuke Kohda Biophysical reviews . 2018,第2期

机译：蛋白质结合位点中的“动态平衡中的多次部分识别形成了结构多样化配体的混杂识别的分子基础
2. Binding specificity and mRNA targets of a C. elegans PUF protein, FBF-1. [J] . Bernstein D, Hook B, Hajarnavis A, RNA . 2005,第4期

机译：秀丽隐杆线虫PUF蛋白FBF-1的结合特异性和mRNA靶标。
3. Structural Basis for the Recognition of Cellular mRNA Export Factor REF by Herpes Viral Proteins HSV-1 ICP27 and HVS ORF57 [J] . Adrian Whitehouse, Alexander P. Golovanov, Brian R. Jackson, PLoS Pathogens . 2011,第1期

机译：疱疹病毒蛋白HSV-1 ICP27和HVS ORF57对细胞mRNA出口因子Ref识别细胞mRNA出口因子Ref的结构依据
4. OPTIMIZING A BACTERIAL sRNA SCAFFOLD FOR TARGETING MULTIPLE mRNAS, FILTERING OFF- TARGET mRNA INTERACTIONS, AND BALANCING METABOLIC PATHWAY FLUX [C] . Richard A. Lease, Samuel D. Stimple, Ashwin Lahiry, Biochemical and molecular engineering XX: the next generation of biochemical engineering: from nanoscale to industrial scale . 2017

机译：优化用于靶向多种mRNA的细菌sRNA支架，过滤脱靶mRNA相互作用以及平衡代谢途径通量
5. Puf1p-mediated mRNA decay and combinatorial control of mRNA stability by the yeast Puf proteins [D] . Ulbricht, Randi J. 2008

机译：Puf1p介导的酵母Puf蛋白降解和mRNA稳定性的组合控制
6. Structural basis for specific recognition of multiple mRNA targets by a PUF regulatory protein [O] . Yeming Wang, Laura Opperman, Marvin Wickens, 2009

机译：PUF调节蛋白特异性识别多个mRNA靶标的结构基础
7. Structural basis for specific recognition of multiple mRNA targets by a PUF regulatory protein [O] . Wang, Yeming, Opperman, Laura, Wickens, Marvin, 2009

机译：PUF调节蛋白特异性识别多个mRNA靶标的结构基础

Structural basis for specific recognition of multiple mRNA targets by a PUF regulatory protein

摘要

著录项

相似文献

相关主题

期刊订阅