...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Alternative splicing and gene duplication differentially shaped the regulation of isochorismate synthase in Populus and Arabidopsis
【24h】

Alternative splicing and gene duplication differentially shaped the regulation of isochorismate synthase in Populus and Arabidopsis

机译：选择性剪接和基因复制不同程度地影响了胡杨和拟南芥中异戊酸合酶的调控

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Isochorismate synthase (ICS) converts chorismate to isochorismate for the biosynthesis of phylloquinone, an essential cofactor for photosynthetic electron transport. ICS is also required for salicylic acid (SA) synthesis during Arabidopsis defense. In several other species, including Populus, SA is derived primarily from the phe-nylpropanoid pathway. We therefore sought to investigate ICS regulation in Populus to learn the extent of ICS involvement in SA synthesis and defense. Arabidopsis harbors duplicated AtlCS genes that differ in their exon-intron structure, basal expression, and stress inducibility. In contrast, we found a single ICS gene in Populus and six other sequenced plant genomes, pointing to the AtlCS duplication as a lineage-specific event. The Populus ICS encodes a functional plastidic enzyme, and was not responsive to stresses that stimulated phenylpropanoid accumulation. Populus ICS underwent extensive alternative splicing that was rare for the duplicated AtlCSs. Sequencing of 184 RT-PCR Populus clones revealed 37 alternative splice variants, with normal transcripts representing ≈50% of the population. When expressed in Arabidopsis, Populus ICS again underwent alternative splicing, but did not produce normal transcripts to complement AtlCS1 function. The splice-site sequences of Populus ICS are unusual, suggesting a causal link between junction sequence, alternative splicing, and ICS function. We propose that gene duplication and alternative splicing of ICS evolved independently in Arabidopsis and Populus in accordance with their distinct defense strategies. AtlCS1 represents a divergent isoform for inducible SA synthesis during defense. Populus ICS primarily functions in phylloquinone biosynthesis, a process that can be sustained at low ICS transcript levels.
机译：异氰酸酯合酶（ICS）将分支酸酯转化为异邻苯二甲酸酯，以进行叶绿醌的生物合成，叶绿醌是光合作用电子转运的重要辅助因子。拟南芥防御过程中水杨酸（SA）的合成也需要ICS。在包括胡杨在内的其他几个物种中，SA主要来自苯丙基丙烷途径。因此，我们寻求调查胡杨中的ICS法规，以了解ICS参与SA合成和防御的程度。拟南芥包含重复的AtlCS基因，它们的外显子-内含子结构，基础表达和应激诱导性不同。相反，我们在胡杨和其他六个已测序植物基因组中发现了一个ICS基因，指出At​​lCS复制是一种谱系特异性事件。杨ICS编码功能性质体酶，并且对刺激苯丙烷积累的压力无反应。 Populus ICS经历了广泛的替代剪接，这对于重复的AtlCS而言是罕见的。 184个RT-PCR杨树克隆的测序揭示了37个替代剪接变体，其中正常转录本占种群的约50％。当在拟南芥中表达时，Populus ICS再次经历了选择性剪接，但没有产生正常的转录本来补充AtlCS1功能。胡杨ICS的剪接位点序列是不寻常的，表明连接序列，替代剪接和ICS功能之间存在因果关系。我们建议基因复制和ICS的选择性剪接根据其独特的防御策略在拟南芥和胡杨中独立进化。 AtlCS1代表防御过程中可诱导的SA合成的不同同工型。胡杨ICS主要在叶醌生物合成中起作用，该过程可以在低ICS转录本水平下维持。

著录项

  • 来源
  • 作者

    Yinan Yuan; Jeng-Der Chung; Xueyan Fu; Virgil E. Johnson; Priya Ranjan; Sarah L. Booth; Scott A. Harding; Chung-Jui Tsai;

  • 作者单位

    School of Forest Resources and Environmental Science, Michigan Technological University, Houghton, Ml 49931;

    School of Forestry and Natural Resources, University of Georgia, Athens, GA 30602 Division of Silviculture, Taiwan Forestry Research Institute, Taipei, Taiwan;

    Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111;

    School of Forestry and Natural Resources, University of Georgia, Athens, GA 30602;

    Oak Ridge National Laboratory, Oak Ridge, TN 37831;

    Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111;

    School of Forest Resources and Environmental Science, Michigan Technological University, Houghton, Ml 49931 School of Forestry and Natural Resources, University of Georgia, Athens, GA 30602;

    School of Forest Resources and Environmental Science, Michigan Technological University, Houghton, Ml 49931 School of Forestry and Natural Resources, University of Georgia, Athens, GA 30602 Department of Genetics, University of Georgia, Athens, GA 30602;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    defense; phenylpropanoid; splice site sequence; phylloquinone; salicylic acid;

    机译：防御;苯丙烷;剪接位点序列;叶醌水杨酸;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号