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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Whole-genome comparison of disease and carriage strains provides insights into virulence evolution in Neisseria meningitidis
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Whole-genome comparison of disease and carriage strains provides insights into virulence evolution in Neisseria meningitidis

机译：疾病和携带菌株的全基因组比较提供了对脑膜炎奈瑟氏球菌毒力进化的见解

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摘要

Neisseria meningitidis is a leading cause of infectious childhood mortality worldwide. Most research efforts have hitherto focused on disease isolates belonging to only a few hypervirulent clonal lineages. However, up to 10% of the healthy human population is temporarily colonized by genetically diverse strains mostly with little or no pathogenic potential. Currently, little is known about the biology of carriage strains and their evolutionary relationship with disease isolates. The expression of a polysaccharide capsule is the only trait that has been convincingly linked to the pathogenic potential of N. meningitidis. To gain insight into the evolution of virulence traits in this species, whole-genome sequences of three meningococcal carriage isolates were obtained. Gene content comparisons with the available genome sequences from three disease isolates indicate that there is no core pathogenome in N. meningitidis. A comparison of the chromosome structure suggests that a filamentous prophage has mediated large chromosomal rearrangements and the translocation of some candidate virulence genes. Interspecific comparison of the available Neisseria genome sequences and dot blot hybridizations further indicate that the insertion sequence IS16S5 is restricted only to N. meningitidis) its low sequence diversity is an indicator of an evolutionary recent population bottleneck. A genome-based phylogenetic reconstruction provides evidence that N. meningitidis has emerged as an unencapsulated human commensal from a common ancestor with Neisseria gonorrhoeae and Neisseria lactamica and consecutively acquired the genes responsible for capsule synthesis via horizontal gene transfer.
机译：脑膜炎奈瑟氏球菌是全世界儿童期传染性死亡的主要原因。迄今为止，大多数研究工作都集中在仅属于少数高毒力克隆谱系的疾病分离株上。但是，多达10％的健康人群被遗传变异的菌株暂时定居，这些菌株大多没有或根本没有致病性。目前，关于运输菌株的生物学及其与疾病分离株的进化关系知之甚少。多糖胶囊的表达是唯一令人信服地与脑膜炎奈瑟氏球菌致病性相关的特征。为了深入了解该物种毒力性状的进化，获得了三个脑膜炎球菌载体分离株的全基因组序列。基因含量与来自三种疾病分离株的可用基因组序列的比较表明，脑膜炎奈瑟氏球菌中没有核心病原体组。染色体结构的比较表明，丝状噬菌体介导了较大的染色体重排和某些候选毒力基因的易位。可用的奈瑟氏球菌基因组序列和斑点印迹杂交的种间比较进一步表明，插入序列IS16S5仅限于脑膜炎奈瑟菌。其低序列多样性是近期进化瓶颈的一个指标。基于基因组的系统发育重建提供的证据表明，脑膜炎奈瑟氏球菌已从淋病奈瑟氏球菌和乳酸奈瑟氏球菌的共同祖先以未包囊的人类共生的形式出现，并通过水平基因转移连续获取了负责胶囊合成的基因。

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