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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >MLST of housekeeping genes captures geographic population structure and suggests a European origin of Borrelia burgdorferi
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MLST of housekeeping genes captures geographic population structure and suggests a European origin of Borrelia burgdorferi

机译：持家基因的MLST捕获了地理种群结构，并暗示欧洲起源的疏螺旋体

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摘要

Lyme borreliosis, caused by the tick-borne bacterium Borrelia burgdorferi. has become the most common vector-borne disease in North America over the last three decades. To understand the dynamics of the epizootic spread and to predict the evolutionary trajectories of B. burgdorferi, accurate information on the population structure and the evolutionary relationships of the pathogen is crucial. We, therefore, developed a multilocus sequence typing (MLST) scheme for B. burgdorferi based on eight chromosomal housekeeping genes. We validated the MLST scheme on B. burgdorferi specimens from North America and Europe, comprising both cultured isolates and infected ticks. These data were compared with sequences for the commonly used genetic markers rrs-rrlA intergenic spacer (IGS) and the gene encoding the outer surface protein C (ospC). The study demonstrates that the concatenated sequences of the housekeeping genes of B. burgdorferi provide highly resolved phylogenetic signals and that the housekeeping genes evolve differently compared with the IGS locus and ospC. Using sequence data, the study reveals that North American and European populations of B. burgdorferi correspond to genetically distinct populations. Importantly, the MLST data suggest that B. burgdorferi originated in Europe rather than in North America as proposed previously.
机译：由the传细菌伯氏疏螺旋体引起的莱姆疏螺旋体病。在过去的三十年中，它已成为北美最常见的媒介传播疾病。为了了解流行病传播的动态并预测B. burgdorferi的进化轨迹，关于种群结构和病原体进化关系的准确信息至关重要。因此，我们基于8个染色体看家基因开发了一种用于B. burgdorferi的多基因座序列分型（MLST）方案。我们验证了来自北美和欧洲的伯氏疏螺旋体标本的MLST方案，包括培养的分离株和受感染的s。将这些数据与常用遗传标记rrs-rrlA基因间隔子（IGS）的序列和编码外表面蛋白C（ospC）的基因进行比较。该研究表明，B。burgdorferi管家基因的串联序列提供了高度解析的系统发生信号，并且管家基因与IGS基因座和ospC的进化方式不同。利用序列数据，该研究揭示了北美和欧洲的伯氏疏螺旋体种群与遗传上不同的种群相对应。重要的是，MLST数据表明B. burgdorferi起源于欧洲，而不是先前提出的北美。

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