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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Outer-membrane transport of aromatic hydrocarbons as a first step in biodegradation
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Outer-membrane transport of aromatic hydrocarbons as a first step in biodegradation

机译：芳香烃的外膜运输是生物降解的第一步

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摘要

Bacterial biodegradation of hydrocarbons, an important process for environmental remediation, requires the passage of hydropho-bic substrates across the cell membrane. Here, we report crystal structures of two outer membrane proteins, Pseudomonas putida TodX and Ralstonia pickettii TbuX, which have been implicated in hydrocarbon transport and are part of a subfamily of the FadL fatty acid transporter family. The structures of TodX and TbuX show significant differences with those previously determined for Escherichia coli FadL, which may provide an explanation for the substrate-specific transport of TodX and TbuX observed with in vivo transport assays. The TodX and TbuX structures revealed 14-stranded β-barrels with an N-terminal hatch domain blocking the barrel interior. A hydrophobic channel with bound detergent molecules extends from the extracellular surface and is contiguous with a passageway through the hatch domain, lined by both hydrophobic and polar or charged residues. The TodX and TbuX structures support a mechanism for transport of hydrophobic substrates from the extracellular environment to the periplasm via a channel through the hatch domain.
机译：碳氢化合物的细菌生物降解是环境修复的重要过程，需要疏水性底物穿过细胞膜。在这里，我们报告了两个外膜蛋白，假单胞菌假单胞菌TodX和Ralstonia pickettii TbuX的晶体结构，它们与碳氢化合物的运输有关，并且是FadL脂肪酸转运蛋白家族的一个子家族的一部分。 TodX和TbuX的结构与先前对大肠杆菌FadL确定的结构显示出显着差异，这可能为在体内转运分析中观察到的TodX和TbuX的底物特异性转运提供了解释。 TodX和TbuX结构揭示了14链β桶，其N端孵化域阻塞了桶的内部。具有结合的去污剂分子的疏水性通道从细胞外表面延伸，并与穿过孵化域的通道相邻，该通道由疏水性和极性或带电残基衬里。 TodX和TbuX结构支持一种机制，用于将疏水性底物通过穿过孵化域的通道从细胞外环境转运至周质。

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