• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >A positive-feedback mechanism promotes reovirus particle conversion to the intermediate associated with membrane penetration
【24h】

A positive-feedback mechanism promotes reovirus particle conversion to the intermediate associated with membrane penetration

机译：正反馈机制促进呼肠孤病毒颗粒转化为与膜渗透相关的中间体

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Membrane penetration by reovirus is associated with conversion of a metastable intermediate, the ISVP, to a further-disassembled particle, the ISVP~*. Factors that promote this conversion in cells are poorly understood. Here, we report the in vitro characterization of a positive-feedback mechanism for promoting ISVP~* conversion. At high particle concentration, conversion approximated second-order kinetics, and products of the reaction operated in trans to promote the conversion of target ISVPs. Pore-forming peptide μ1 N, which is released from particles during conversion, was sufficient for promoting activity. A mutant that does not undergo μ1N release failed to exhibit second-order conversion kinetics and also failed to promote conversion of wild-type target ISVPs. Susceptibility of target ISVPs to promotion in trans was temperature dependent and correlated with target stability, suggesting that capsid dynamics are required to expose the interacting epitope. A positive-feedback mechanism of promoting escape from the metastable intermediate has not been reported for other viruses but represents a generalizable device for sensing a confined volume, such as that encountered during cell entry.
机译：呼肠孤病毒的膜渗透与亚稳中间体ISVP转化为进一步分解的颗粒ISVP〜*有关。促进细胞中这种转化的因素了解甚少。在这里，我们报告了促进ISVP〜*转化的正反馈机制的体外表征。在高颗粒浓度下，转化率接近二级动力学，反应产物反式操作以促进目标ISVP的转化。在转化过程中从颗粒释放的成孔肽μ1N足以促进活性。未经历μ1N释放的突变体无法显示二级转化动力学，也无法促进野生型靶标ISVP的转化。靶标ISVPs在反式中的易感性是温度依赖性的，并且与靶标稳定性相关，表明需要衣壳动力学来暴露相互作用的表位。对于其他病毒，尚未报道过促进亚稳中间体逃逸的正反馈机制，但它代表了一种可感测受限体积（如细胞进入过程中遇到的体积）的通用设备。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号