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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >D-retrovirus morphogenetic switch driven by the targeting signal accessibility to Tctex-1 of dynein
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D-retrovirus morphogenetic switch driven by the targeting signal accessibility to Tctex-1 of dynein

机译：D-逆转录病毒形态发生开关由动力蛋白对Tctex-1的靶向信号可及性驱动

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摘要

Despite extensive data demonstrating that immature retroviral particle assembly can take place either at the plasma membrane or at a distinct location within the cytoplasm, targeting of viral precursor proteins to either assembly site still remains poorly understood. Biochemical data presented here suggest that Tctex-1, a light chain of the molecular motor dynein, is involved in the intracellular targeting of Mason-Pfizer monkey virus (M-PMV) polyproteins to the cytoplasmic assembly site. Comparison of the three-dimensional structures of M-PMV wild-type matrix protein (wt MA) with a single amino acid mutant (R55F), which redirects assembly from a cytoplasmic site to the plasma membrane, revealed different mutual orientations of their C- and N-terminal domains. This conformational change buries a putative intracellular targeting motif located between both domains in the hydro-phobic pocket of the MA molecule, thereby preventing the interaction with cellular transport mechanisms.
机译：尽管有大量数据表明，不成熟的逆转录病毒颗粒装配可以在质膜上或在细胞质内的不同位置发生，但将病毒前体蛋白靶向任一装配位点仍知之甚少。这里介绍的生化数据表明，Tctex-1（分子动力达因的轻链）参与了梅森-辉瑞猴病毒（M-PMV）多蛋白向细胞质装配位点的细胞内靶向。 M-PMV野生型基质蛋白（wt MA）与单个氨基酸突变体（R55F）的三维结构比较，该突变体将装配从胞质位点重定向到质膜，揭示了它们C-的互不相同方向和N末端域。该构象变化掩盖了位于MA分子的疏水口袋中的两个结构域之间的推定细胞内靶向基序，从而防止了与细胞转运机制的相互作用。

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