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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Combinatorial surrobody libraries
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Combinatorial surrobody libraries

机译：组合替代体库

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摘要

A unique type of combinatorial protein libraries has been constructed. These libraries are based on the pre-B cell receptor (pre-BCR). The pre-BCR is a protein that is produced during normal development of the antibody repertoire. Unlike that of canonical antibodies, the pre-BCR subunit is a trimer that is composed of an antibody heavy chain paired with two surrogate light chain (SLC) components. Combinatorial libraries based on these pre-BCR proteins in which diverse heavy chains are paired with a fixed SLC were expressed in mammalian, Escherichia coli, and phagemid systems. These libraries contain members that have nanomolar affinity for antigen. We term this type of antigen-binding protein a "surrobody" to distinguish it from the canonical antibody molecule.
机译：已经构建了独特类型的组合蛋白文库。这些库基于前B细胞受体（pre-BCR）。 BCR前体是在抗体库正常发育过程中产生的蛋白质。与标准抗体不同，前BCR亚基是三聚体，由抗体重链和两个替代轻链（SLC）组成。在哺乳动物，大肠杆菌和噬菌粒系统中表达了基于这些pre-BCR蛋白的组合文库，其中多种重链与固定的SLC配对。这些文库包含对抗原具有纳摩尔摩尔亲和力的成员。我们将这种类型的抗原结合蛋白称为“替代抗体”，以将其与标准抗体分子区分开。

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