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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Electric Field-induced Direct Delivery Of Proteins By A Nanofountain Probe
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Electric Field-induced Direct Delivery Of Proteins By A Nanofountain Probe

机译：纳米喷泉探针对电场的蛋白质直接递送

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摘要

We report nanofabrication of protein dot and line patterns using a nanofountain atomic force microscopy probe (NFP). Biomolecules are continuously fed in solution through an integrated microfluidic system, and deposited directly onto a substrate. Deposition is controlled by application of an electric potential of appropriate sign and magnitude between the probe reservoir and substrate. Submicron dot and line molecular patterns were generated with resolution that depended on the magnitude of the applied voltage, dwell time, and writing speed. By using an energetic argument and a Kelvin condensation model, the quasi-equilibrium liquid-air interface at the probe tip was determined. The analysis revealed the origin of the need for electric fields in achieving protein transport to the substrate and confirmed experimental observations suggesting that pattern resolution is controlled by tip sharpness and not overall probe aperture. As such, the NFP combines the high-resolution of dip-pen nanolithography with the efficient continuous liquid feeding of micropipettes while allowing scalability to 1-and 2D probe arrays for high throughput.
机译：我们报告使用纳米喷泉原子力显微镜探针（NFP）的蛋白质点和线图案的纳米加工。通过集成的微流体系统将生物分子连续注入溶液中，并直接沉积在基质上。沉积是通过在探针容器和基板之间施加适当符号和大小的电势来控制的。产生的亚微米点和线分子图案的分辨率取决于所施加电压的大小，停留时间和写入速度。通过使用高能参数和开尔文冷凝模型，确定了探针尖端的准平衡液-气界面。该分析揭示了实现蛋白质转运至底物所需电场的起源，并证实了实验观察结果，表明图案分辨率是由尖端的锐度而不是整个探针的孔径控制的。这样，NFP将浸笔式纳米光刻的高分辨率与微量移液器的高效连续液体进料相结合，同时可扩展至一维和二维探头阵列，以实现高通量。

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