...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Tissue-specific activity of the blind mole rat and the two nucleotide-mutated mouse αB-crystallin promoter in transgenic mice
【24h】

Tissue-specific activity of the blind mole rat and the two nucleotide-mutated mouse αB-crystallin promoter in transgenic mice

机译：盲mole鼠和两个核苷酸突变的小鼠αB-晶状体蛋白启动子在转基因小鼠中的组织特异性活性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The αB-crystallin and HspB2 genes are located 0.9 kb apart in a head-to-head arrangement in mammals. Previous experiments have shown that a truncated -668/+45 αB-crystallin enhancer/ promoter fragment from blind mole rats (Spa/ax ehrenbergi), which have nonfunctional lenses, lacks lens activity and has enhanced muscle activity in transgenic mice. Here we show that the full-length mole rat αB-crystallin intergenic region behaves similarly in transgenic mice. A two-nucleotide mutation (~(-273)CA→G) in the mouse αB-crystallin enhancer/promoter fragment mimicking the wild-type mole rat sequence functionally converted the mouse promoter fragment to that of the wild-type mole rat promoter when tested in transgenic mice. The reciprocal mutation in the mole rat promoter fragment (~(-272)G→CA) did not affect its activity. Oligonucleotides from the wild-type mouse and mole rat αB-crystallin promoter region under study formed distinct complexes with nuclear proteins from cultured cells. The mouse mutant sequence lost binding ability, whereas the mutated mole rat sequence gained the ability to form a complex similar in size to that of the wild-type mouse oligonucleotide. Our data support the idea that blind mole rats' αB-crystallin promoter activity was modified during the evolution of subterranean life and shows that tissue-specific promoter activity can be modulated by changing as few as two apparently neutral nucleotides in the mouse αB-crystallin enhancer region, implying the importance of the context of regulatory sequences for promoter activity.
机译：在哺乳动物中，头对头排列的αB-晶状蛋白和HspB2基因相距0.9 kb。先前的实验表明，来自盲mole鼠（Spa / ax ehrenbergi）的截断的-668 / + 45αB-晶状蛋白增强子/启动子片段具有无功能的晶状体，缺乏晶状体活性，并且在转基因小鼠中具有增强的肌肉活性。在这里，我们显示全长摩尔大鼠αB-crystallin基因间区域在转基因小鼠中表现相似。模仿野生型mole鼠大鼠序列的小鼠αB-晶状蛋白增强子/启动子片段中的两个核苷酸突变（〜（-273）CA→G）在功能上将小鼠启动子片段转化为野生型mole鼠大鼠启动子片段在转基因小鼠中测试。 mole鼠大鼠启动子片段（〜（-272）G→CA）中的相互突变不影响其活性。来自所研究的野生型小鼠和mole鼠αB-crystallin启动子区域的寡核苷酸与培养细胞的核蛋白形成了独特的复合物。小鼠突变体序列失去了结合能力，而突变的mole鼠大鼠序列获得了形成大小与野生型小鼠寡核苷酸相似的复合物的能力。我们的数据支持这样的想法，即在地下动物的进化过程中改变了盲鼠的αB-晶状体蛋白启动子活性，并表明可以通过改变小鼠αB-晶状体蛋白增强子中至少两个明显的中性核苷酸来调节组织特异性启动子活性。区域，暗示调控序列的背景对于启动子活性的重要性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号