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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >DgrA is a member of a new family of cyclic diguanosine monophosphate receptors and controls flagellar motor function in Caulobacter crescentus
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DgrA is a member of a new family of cyclic diguanosine monophosphate receptors and controls flagellar motor function in Caulobacter crescentus

机译：DgrA是新的环状双鸟嘌呤单磷酸受体家族的成员，并控制新月形杆菌中的鞭毛运动功能

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摘要

Bacteria are able to switch between two mutually exclusive lifestyles, motile single cells and sedentary multicellular communities that colonize surfaces. These behavioral changes contribute to an increased fitness in structured environments and are controlled by the ubiquitous bacterial second messenger cyclic diguanosine monophosphate (c-di-GMP). In response to changing environments, fluctuating levels of c-di-GMP inversely regulate cell motil-ity and cell surface adhesins. Although the synthesis and breakdown of c-di-GMP has been studied in detail, little is known about the downstream effector mechanisms. Using affinity chromatog-raphy, we have isolated several c-di-GMP-binding proteins from Caulobacter crescentus. One of these proteins, DgrA, is a PilZ homolog involved in mediating c-di-GMP-dependent control of C. crescentus cell motility. Biochemical and structural analysis of DgrA and homologs from C. crescentus, Salmonella typhimurium, and Pseudomonas aeruginosa demonstrated that this protein family represents a class of specific diguanylate receptors and suggested a general mechanism for c-di-GMP binding and signal transduction. Increased concentrations of c-di-GMP or DgrA blocked motility in C. crescentus by interfering with motor function rather than flagellar assembly. We present preliminary evidence implicating the flagellar motor protein FliL in DgrA-dependent cell motility control.
机译：细菌能够在两种相互排斥的生活方式，活动的单细胞和定居在表面的久坐的多细胞群落之间切换。这些行为改变有助于在结构化环境中提高适应度，并受到普遍存在的细菌第二信使环二鸟苷单磷酸（c-di-GMP）的控制。响应不断变化的环境，c-di-GMP水平的波动反过来调节细胞运动性和细胞表面粘附素。尽管已经详细研究了c-di-GMP的合成和分解，但对下游效应子机制了解甚少。使用亲和层析，我们从新月形杆菌中分离了几种c-di-GMP结合蛋白。这些蛋白之一DgrA是参与介导c-di-GMP依赖的C.crescentus细胞运动的PilZ同源物。生化和结构分析DgrA和C.crescentus，鼠伤寒沙门氏菌和铜绿假单胞菌的同源物表明，该蛋白家族代表一类特定的双鸟苷酸受体，并提出了c-di-GMP结合和信号转导的一般机制。 c-di-GMP或DgrA浓度的升高通过干扰运动功能而不是鞭毛组装来阻止新月形梭菌的运动。我们目前初步证据牵连鞭毛运动蛋白FliL在依赖DgrA的细胞运动控制中。

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