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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Gap junctions on hippocampal mossy fiber axons demonstrated by thin-section electron microscopy and freeze-fracture replica immunogold labeling
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Gap junctions on hippocampal mossy fiber axons demonstrated by thin-section electron microscopy and freeze-fracture replica immunogold labeling

机译：薄层电子显微镜和冷冻断裂复制免疫金标记显示海马苔藓纤维轴突上的缝隙连接

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摘要

Gap junctions have been postulated to exist between the axons of excitatory cortical neurons based on electrophysiological, modeling, and dye-coupling data. Here, we provide ultrastructural evidence for axoaxonic gap junctions in dentate granule cells. Using combined confocal laser scanning microscopy, thin-section transmission electron microscopy, and grid-mapped freeze-fracture replica immunogold labeling, 10 close appositions revealing axoaxonic gap junctions (≈30-70 nm in diameter) were found between pairs of mossy fiber axons ( ≈100-200 nm in diameter) in the stratum lucidum of the CA3b field of the rat ventral hippocampus, and one axonal gap junction (≈100 connexons) was found on a mossy fiber axon in the CA3c field of the rat dorsal hippocampus. Immunogold labeling with two sizes of gold beads revealed that connexin36 was present in that axonal gap junction. These ultrastructural data support computer modeling and in vitro electrophysiological data suggesting that axoaxonic gap junctions play an important role in the generation of very fast (>70 Hz) network oscillations and in the hypersynchronous electrical activity of epilepsy.
机译：基于电生理，建模和染料耦合数据，已假定在兴奋性皮质神经元的轴突之间存在间隙连接。在这里，我们提供了齿状颗粒细胞中轴突间隙连接的超微结构证据。使用组合共焦激光扫描显微镜，薄层透射电子显微镜和网格映射的冷冻断裂复制品免疫金标记，在长满苔藓的纤维轴突对之间发现了10个紧密并列的轴突间隙连接（直径约30-70 nm）。在大鼠腹侧海马CA3b区域的透明细胞中，直径约≈100-200 nm），并且在大鼠背海马CA3c区域的一个长满苔藓的纤维轴突上发现了一个轴突间隙连接处（约100个连接子）。带有两种大小的金珠的免疫金标记表明，轴突间隙连接处存在连接蛋白36。这些超微结构数据支持计算机建模和体外电生理数据，表明轴突间隙连接在快速（> 70 Hz）网络振荡的产生和癫痫的超同步电活动中起着重要作用。

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