...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Increased motion and travel, rather than stable docking, characterize the last moments before secretory granule fusion
【24h】

Increased motion and travel, rather than stable docking, characterize the last moments before secretory granule fusion

机译：增加的运动和行程而不是稳定的对接，是分泌性颗粒融合之前的最后时刻

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The state of secretory granules immediately before fusion with the plasma membrane is unknown, although the granules are generally assumed to be stably bound (docked). We had previously developed methods using total internal reflection fluorescence microscopy and image analysis to determine the position of chro-maffin granules immediately adjacent to the plasma membrane with high precision, often to within ≈10 nm, or < 5% of the granule diameter (300 nm). These distances are of the dimensions of large proteins and are comparable with the unitary step sizes of molecular motors. Here we demonstrate with quantitative measures of granule travel in the plane parallel to the plasma membrane that secretory granules change position within several hundred milliseconds of nicotinic agonist-induced fusion. Furthermore, just before fusion, granules frequently move to areas that they have rarely visited. The movement of granules to new areas is most evident for granules that fuse later during the stimulus. The movement may increase the probability of productive interactions of the granule with the plasma membrane or may reflect the pull of molecular interactions between the granule and the plasma membrane that are part of the fusion process. Thus, instead of being stably docked before exocytosis, granules undergo molecular-scale motions and travel immediately preceding the fusion event.
机译：尽管通常认为这些颗粒是稳定结合的（对接的），但在与质膜融合之前，分泌颗粒的状态尚不清楚。我们之前已经开发出了使用全内反射荧光显微镜和图像分析的方法，以高精度确定通常邻近质膜约10 nm或小于颗粒直径5％（300微米）的铬-石蜡颗粒的位置纳米）。这些距离是大蛋白的尺寸，可与分子马达的单位步长相媲美。在这里，我们用定量测量的方法证明了在平行于质膜的平面中颗粒的运动，分泌颗粒在烟碱激动剂诱导的融合作用的几百毫秒内改变了位置。此外，在融合之前，颗粒经常移动到他们很少去过的区域。对于在刺激期间稍后融合的颗粒而言，颗粒向新区域的运动最为明显。该运动可以增加颗粒与质膜的有效相互作用的可能性，或者可以反映作为融合过程的一部分的颗粒与质膜之间的分子相互作用的拉动。因此，不是在胞吐作用之前稳定地停靠，而是颗粒经历分子尺度的运动并且在融合事件之前立即行进。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号