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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Microtubule-based nuclear movement occurs independently of centrosome positioning in migrating neurons
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Microtubule-based nuclear movement occurs independently of centrosome positioning in migrating neurons

机译：基于微管的核运动独立于迁移神经元中的中心体定位而发生

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摘要

During neuronal migration in the developing brain, it is thought that the centrosome precedes the nucleus and provides a cue for nuclear migration along the microtubules. In time-lapse imaging studies of radially migrating granule cells in mouse cerebellar slices, we observed that the movements of the nucleus and centrosome appeared to occur independently of each other. The nucleus often migrated ahead of the centrosome during its saltatory movement, negating the supposed role of the centrosome in pulling the nucleus. The nucleus was associated with dynamic microtubules enveloping the entire nucleus and stable microtubules extending from the leading process to the anterior part of the nucleus. Neither of these perinuclear microtubules converged at the centrosome. Disruption or excess formation of stable microtubules attenuated nuclear migration, indicating that the configuration of stable microtubules is crucial for nuclear migration. The inhibition of LIS1 function, a regulator of a microtubule motor dynein, specifically blocks nuclear migration without affecting the coupling of the centrosome and microtubules in the leading process, suggesting that movements of the nucleus and centrosome are differentially regulated by dynein motor function. Thus, the nucleus moves along the microtubules independently of the position of the centrosome in migrating neurons.
机译：在发育中的大脑神经元迁移过程中，人们认为中心体先于细胞核，为沿着微管的细胞核迁移提供了线索。在小鼠小脑切片中径向迁移颗粒细胞的延时成像研究中，我们观察到核和中心体的运动似乎是相互独立发生的。核在盐运动过程中经常迁移到中心体之前，从而抵消了中心体在拉动核中的假定作用。核与包裹整个核的动态微管和从前突延伸至核前部的稳定微管有关。这些核周微管都没有在中心体处汇聚。稳定微管的破坏或过度形成减弱了核迁移，这表明稳定微管的构型对于核迁移至关重要。对LIS1功能的抑制是微管马达动力蛋白的调节剂，它特别阻止核的迁移，而不会影响中心体与微管在前导过程中的偶联，这表明核素和中心体的运动受动力元件的运动调节。因此，细胞核沿着微管移动，而与中心体在迁移神经元中的位置无关。

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