Prestressed F-actin networks cross-linked by hinged filamins replicate mechanical properties of cells

M. L. Garden; F. Nakamura; J. H. Hartwig; J. C. Crocker; T. P. Stossel; D. A. Weitz

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Prestressed F-actin networks cross-linked by hinged filamins replicate mechanical properties of cells

机译：通过铰链丝蛋白交联的预应力F-肌动蛋白网络复制了细胞的机械性能

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摘要

We show that actin filaments, shortened to physiological lengths by gelsolin and cross-linked with recombinant human filamins (FLNs), exhibit dynamic elastic properties similar to those reported for live cells. To achieve elasticity values of comparable magnitude to those of cells, the in vitro network must be subjected to external prestress, which directly controls network elasticity. A molecular requirement for the strain-related behavior at physiological conditions is a flexible hinge found in FLNa and some FLNb molecules. Basic physical properties of the in vitro filamin-F-actin network replicate the essential mechanical properties of living cells. This physical behavior could accommodate passive deformation and internal organelle trafficking at low strains yet resist externally or internally generated high shear forces.

机译：我们显示肌动蛋白丝，通过凝溶胶蛋白缩短到生理长度，并与重组人丝素蛋白（FLNs）交联，表现出类似于活细胞报道的动态弹性特性。为了获得与细胞相当的弹性值，体外网络必须承受外部预应力，该应力直接控制网络弹性。在生理条件下，应变相关行为的分子要求是在FLNa和某些FLNb分子中发现的柔性铰链。体外丝素-F-肌动蛋白网络的基本物理特性复制了活细胞的基本机械特性。这种物理行为可以适应低应变下的被动变形和内部细胞器运输，但可以抵抗外部或内部产生的高剪切力。

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《Proceedings of the National Academy of Sciences of the United States of America》 |2006年第6期|p.1762-1767|共6页
作者
M. L. Garden; F. Nakamura; J. H. Hartwig; J. C. Crocker; T. P. Stossel; D. A. Weitz;
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作者单位

Department of Physics and Division of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
关键词
cytoskeleton; cell mechanics; nonlinear rheology;

机译：细胞骨架;细胞力学;非线性流变学;

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1. Structural and viscoelastic properties of actin/filamin networks: cross-linked versus bundled networks. [J] . Schmoller KM, Lieleg O, Bausch AR Biophysical Journal . 2009,第1期

机译：肌动蛋白/纤维蛋白网络的结构和粘弹性：交联网络与捆绑网络。
2. Three-Dimensional Cross-Linked F-Actin Networks: Relation between Network Architecture and Mechanical Behavior [J] . E. M. Huisman, T. van Dillen, P. R. Onck, Physical review letters . 2007,第20期

机译：三维交联F-Actin网络：网络体系结构与机械行为之间的关系
3. An affine continuum mechanical model for cross-linked F-actin networks with compliant linker proteins [J] . Gerhard A. Holzapfel, Michael J. Unterberger, Ray W. Ogden Journal of the mechanical behavior of biomedical materials . 2014,第Null期

机译：具有顺应性接头蛋白的交联F-肌动蛋白网络的仿射连续力学模型
4. ALTERNATIVE SPLICING FOR MECHANICAL RESILIENCE - THE SOFTENING EFFECT OF FILAMIN'S HINGE [C] . Dennis E. Discher, Colin Johnson ASME Bioengineering Conference . 2007

机译：机械弹性的替代拼接 - 丝绒铰链的软化效果
5. The effects of mechanical forces on alveolar epithelial cells: Keratin intermediate filament network structure, phosphorylation, dynamics and mechanical properties [D] . Sivaramakrishnan, Sivaraj 2006

机译：机械力对肺泡上皮细胞的影响：角蛋白中间丝网络结构，磷酸化，动力学和机械性能
6. Prestressed F-actin networks cross-linked by hinged filamins replicate mechanical properties of cells [O] . M. L. Gardel, F. Nakamura, J. H. Hartwig, 2006

机译：通过铰链丝蛋白交联的预应力F-肌动蛋白网络复制了细胞的机械性能
7. Prestressed F-actin networks cross-linked by hinged filamins replicate mechanical properties of cells [O] . Gardel, M. L., Nakamura, F., Hartwig, J. H., 2006

机译：通过铰链丝蛋白交联的预应力F-肌动蛋白网络复制了细胞的机械性能

Prestressed F-actin networks cross-linked by hinged filamins replicate mechanical properties of cells

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