...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Production of infectious genotype 1a hepatitis C virus (Hutchinson strain) in cultured human hepatoma cells
【24h】

Production of infectious genotype 1a hepatitis C virus (Hutchinson strain) in cultured human hepatoma cells

机译：在培养的人肝癌细胞中生产感染性基因型1a丙型肝炎病毒（Hutchinson株）

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Infections with hepatitis C virus (HCV) are marked by frequent viral persistence, chronic liver disease, and extraordinary viral genetic diversity. Although much has been learned about HCV since its discovery, progress has been slowed by a lack of permissive cell culture systems supporting its replication. Productive infections have been achieved recently with genotype 2a virus, but cirrhosis and liver cancer are typically associated with genotype 1 HCV, which is more prevalent and relatively resistant to IFN therapy. We describe production of infectious genotype 1a HCV in cells trans-fected with synthetic RNA derived from a prototype virus (H77-S). Viral proteins accumulated more slowly in H77-S transfected cells than in cells transfected with genotype 2a (JFH-1) RNA, but substantially more H77-S RNA was secreted into supernatant fluids. Most secreted RNA was noninfectious, banding in isopycnic gradients at a density of 1.04-1.07 gm/cm~3, but infectivity was associated with H77-S particles possessing a density of 1.13-1.14 gm/cm~3. The specific infectivity of H77-S particles (5.4 x 10~4 RNA copies per focus-forming unit) was significantly lower than JFH-1 virus (1.4 x 10~2 RNA copies per focus-forming unit). Infection with either virus was blocked by CD81 antibody. Sera from genotype 1a-infected individuals neutralized H77-S virus, but had little activity against genotype 2a virus, suggesting that these genotypes represent different serotypes. The ability of this genotype 1a virus to infect cultured cells will substantially benefit antiviral and vaccine discovery programs.
机译：丙型肝炎病毒（HCV）感染的特征是频繁的病毒持久性，慢性肝病和非凡的病毒遗传多样性。尽管自从发现HCV以来已经学到了很多东西，但是由于缺乏支持其复制的无用细胞培养系统而使进展减慢了。基因型2a病毒最近已实现生产性感染，但肝硬化和肝癌通常与基因型1 HCV有关，后者更普遍且相对抗IFN治疗。我们描述了用衍生自原型病毒（H77-S）的合成RNA转染的细胞中感染性基因型1a HCV的生产。与转基因2a（JFH-1）RNA转染的细胞相比，在H77-S转染的细胞中病毒蛋白质的积聚速度更慢，但在上清液中却分泌了更多的H77-S RNA。大部分分泌的RNA是非感染性的，以等密度梯度以1.04-1.07 gm / cm〜3的密度带状出现，但感染性与密度为1.13-1.14 gm / cm〜3的H77-S颗粒有关。 H77-S颗粒的特异性感染性（每个焦点形成单位5.4 x 10〜4 RNA拷贝）显着低于JFH-1病毒（每个焦点形成单位1.4 x 10〜2 RNA拷贝）。 CD81抗体阻止了两种病毒的感染。来自基因型1a感染个体的血清中和了H77-S病毒，但对基因型2a病毒几乎没有活性，表明这些基因型代表不同的血清型。此基因型1a病毒感染培养细胞的能力将大大有益于抗病毒和疫苗发现计划。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号