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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Sinorhizobium meliloti bluB is necessary for production of 5,6-dimethylbenzimidazole, the lower ligand of B_(12)
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Sinorhizobium meliloti bluB is necessary for production of 5,6-dimethylbenzimidazole, the lower ligand of B_(12)

机译：melilotiblub中华根瘤菌对于生产5_6-二甲基苯并咪唑是B_（12）的较低配体是必需的

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摘要

An insight into a previously unknown step in B_(12) biosynthesis was unexpectedly obtained through our analysis of a mutant of the symbiotic nitrogen fixing bacterium Sinorhizobium meliloti. This mutant was identified based on its unusually bright fluorescence on plates containing the succinoglycan binding dye calcofluor. The mutant contains a Tn5 insertion in a gene that has not been characterized previously in S. meliloti. The closest known homolog is the bluB gene of Rhodobacter capsulatus, which is implicated in the biosynthesis of B_(12) (cobalamin). The S. meliloti bluB mutant is unable to grow in minimal media and fails to establish a symbiosis with alfalfa, and these defects can be rescued by the addition of vitamin B_(12) (cyanocobalamin) or the lower ligand of cobalamin, 5,6-dimethylbenzimidazole (DMB). Biochemical analysis demonstrated that the bluB mutant does not produce cobalamin unless DMB is supplied. Sequence comparison suggests that BluB is a member of the NADH/flavin mononucleotide (FMN)-dependent nitroreductase family, and we propose that it is involved in the conversion of FMN to DMB.
机译：通过对共生固氮细菌Melinotizobium meliloti突变体的分析，出乎意料地获得了对B_（12）生物合成中先前未知步骤的见解。根据该突变体是基于其在含有琥珀聚糖结合染料钙荧光的板上异常亮的荧光而鉴定的。该突变体在之前未在苜蓿链球菌中表征的基因中包含Tn5插入。最接近的已知同源物是荚膜红细菌的bluB基因，该基因与B_（12）（钴胺素）的生物合成有关。 S. meliloti bluB突变体无法在基本培养基中生长，也无法与苜蓿建立共生关系，这些缺陷可以通过添加维生素B_（12）（cyanocobalamin）或钴胺素​​的下部配体5,6来挽救。 -二甲基苯并咪唑（DMB）。生化分析表明，除非提供DMB，否则bluB突变体不会产生钴胺素。序列比较表明，BluB是NADH /黄素单核苷酸（FMN）依赖性硝基还原酶家族的成员，我们建议它参与FMN向DMB的转化。

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