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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Indoleacetic acid, a product of transferred DNA, inhibits vir gene expression and growth of Agrobacterium tumefaciens C58
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Indoleacetic acid, a product of transferred DNA, inhibits vir gene expression and growth of Agrobacterium tumefaciens C58

机译：吲哚乙酸是转移的DNA的产物，可抑制vir基因的表达和根癌农杆菌C58的生长

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摘要

Agrobacterium tumefaciens induces crown gall tumors by transferring a piece of its tumor-inducing plasmid into plant cells. This transferred DNA encodes the synthesis of indole acetic acid (IAA) and cytokinin, and their overproduction results in tumor formation. The transfer is initiated by a two-component regulatory system, VirA/G recognizing plant signal molecules in the plant rhizosphere and activating a regulon on the tumor-inducing plasmid, which is required for the processing and transfer of DNA and protein. Although a great deal is known about vir gene activation, nothing is known about whether or how the vir gene regulon is inactivated after plant cell transformation. Presumably, just as a mechanism exists for activating the vir gene regulon only when a plant is in the immediate environment, a mechanism should exist for inactivating the same regulon once it has fulfilled its mission to transferred DNA into plant cells. We now show that IAA inactivates vir gene expression by competing with the inducing phenolic compound acetosyringone for interaction with VirA. IAA does not inhibit the vir genes in cells containing a constitutive sensor virA locus, which does not require any signal molecules to become phosphorylated. At higher concentrations, IAA inhibits the growth of Agrobacterium and many other plant-associated bacteria but not the growth of bacteria that occupy other ecological niches. These observations provide the missing link in the cycle of vir gene activation and inactivation.
机译：根癌农杆菌通过将其一部分肿瘤诱导质粒转移到植物细胞中来诱导冠状胆囊肿瘤。转移的DNA编码吲哚乙酸（IAA）和细胞分裂素的合成，它们的过量生产导致肿瘤形成。转移是由两部分的调节系统启动的，VirA / G识别植物根际中的植物信号分子并激活肿瘤诱导质粒上的调节子，这是DNA和蛋白质的加工和转移所必需的。尽管人们对vir基因的激活了解很多，但对于在植物细胞转化后是否或如何使vir基因调节子失活一无所知。据推测，正如仅当植物在附近环境中存在激活vir基因调控因子的机制一样，一旦完成了将DNA转移到植物细胞中的任务，就应该存在使相同调控因子失活的机制。我们现在显示，IAA通过与诱导酚类化合物乙酰丁香酮竞争与VirA的相互作用来灭活vir基因表达。 IAA不会抑制含有组成型传感器virA基因座的细胞中的vir基因，该基因不需要任何信号分子被磷酸化。在较高浓度下，IAA会抑制土壤杆菌和许多其他植物相关细菌的生长，但不会抑制占据其他生态位的细菌的生长。这些观察结果提供了vir基因激活和灭活循环中的缺失环节。

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