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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Progestin is an essential factor for the initiation of the meiosis in spermatogenetic cells of the eel
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Progestin is an essential factor for the initiation of the meiosis in spermatogenetic cells of the eel

机译：孕激素是鳗精子细胞中减数分裂启动的重要因素

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Meiosis is an indispensable process of sexual reproduction. However, detailed information on the regulatory mechanisms that initiate meiosis is not available. Progestins are important steroids regulating final maturation in male and female vertebrates. In male teleosts, it is known that progestin induces spermiation and sperm maturation. However, a role for progestin in early spermatogenesis or meiosis has not yet been described. In this study, we examined the functions of progestin on the initiation of meiosis in male Japanese eel. A natural progestin in teleost fish 17 alpha,20 beta-dihydroxy-4-pregnen-3-one (DHP) and its receptors were present in the testis at an early stage of spermatogenesis. By using an eel testicular culture system, DHP was shown to induce DNA replication in spermatogonia. Although 11-ketotestosterone, a known initiator of spermatogenesis, also stimulated DNA synthesis in spermatogonia, antibodies against DHP prevented DNA replication when added during the period in which meiosis was initiated. DHP treatment also induced the expression of meiosis-specific markers, such as Dmcl and Spo11. Furthermore, Spo11 expression and synaptonemal complexes, specific features of the meiotic prophase, were detected in testicular fragments cultured with DHP in some germ cells that showed morphological characteristics of undifferentiated spermatogonia. We conclude that DHP, a progestin, is an essential factor for the initiation of meiosis.
机译：减数分裂是性繁殖的必不可少的过程。但是，尚无有关引发减数分裂的调控机制的详细信息。孕激素是调节雄性和雌性脊椎动物最终成熟的重要类固醇。已知在雄性硬骨鱼中，孕激素可诱导精子和精子成熟。然而，尚未描述孕激素在早期精子发生或减数分裂中的作用。在这项研究中，我们检查了孕激素在雄性日本鳗鱼减数分裂启动中的功能。硬骨鱼17α，20β-二羟基-4-孕烯-3-酮（DHP）及其受体中的天然孕激素在精子发生的早期阶段存在于睾丸中。通过使用鳗鱼睾丸培养系统，DHP已显示出在精原细胞中诱导DNA复制。尽管11-酮睾酮（已知的精子发生起始剂）也刺激了精原细胞的DNA合成，但在减数分裂开始的时期添加抗DHP的抗体可阻止DNA复制。 DHP处理还诱导了减数分裂特异性标志物（例如Dmcl和Spo11）的表达。此外，在某些生殖细胞中用DHP培养的睾丸片段中检测到了Spo11表达和突触复合物，这是减数分裂前期的特定特征，这些细胞显示出未分化的精原细胞的形态特征。我们得出的结论是，DHP（一种孕激素）是减数分裂启动的必要因素。

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