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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Pannexin 1 in erythrocytes: Function without a gap
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Pannexin 1 in erythrocytes: Function without a gap

机译：红细胞中的Pannexin 1：无间隙的功能

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摘要

ATP is a widely used extracellular signaling molecule. The mechanism of ATP release from cells is presently unresolved and may be either vesicular or channel-mediated. Erythrocytes release ATP in response to low oxygen or to shear stress. In the absence of vesicles, the release has to be through channels. Erythrocytes do not form gap junctions. Yet, here we show with immunohistochemical and electrophysiological data that erythrocytes express the gap junction protein pannexin 1. This protein, in addition to forming gap junction channels in paired oocytes, can also form a mechanosensitive and ATP-permeable channel in the nonjunctional plasma membrane. Consistent with a role of pannexin 1 as an ATP release channel, ATP release by erythrocytes was attenuated by the gap junction blocker carbenoxcilone. Furthermore, under conditions of ATP release, erythrocytes took up fluorescent tracer molecules permeant to gap junction channels.
机译：ATP是一种广泛使用的细胞外信号分子。 ATP从细胞释放的机制目前尚未解决，可能是水泡或通道介导的。红细胞响应低氧或剪切应力释放ATP。在没有囊泡的情况下，释放必须通过通道进行。红细胞不形成间隙连接。然而，在这里我们用免疫组织化学和电生理学数据表明红细胞表达了间隙连接蛋白pannexin1。该蛋白除了在成对的卵母细胞中形成间隙连接通道外，还可以在非连接质膜上形成机械敏感性和ATP渗透通道。与pannexin 1作为ATP释放通道的作用一致，间隙连接阻滞剂羧苄西酮减弱了红细胞的ATP释放。此外，在ATP释放的条件下，红细胞吸收了渗透到间隙连接通道的荧光示踪分子。

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