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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Barttin modulates trafficking and function of ClC-K channels
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Barttin modulates trafficking and function of ClC-K channels

机译：Barttin调节ClC-K通道的运输和功能

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摘要

Barttin is an accessory subunit of a subgroup of CIC-type chloride channels expressed in renal and inner ear epithelia. In this study, we examined the effects of barttin on two CIC-K channel isoforms, rat CIC-K1 and human CIC-Kb, using heterologous expression, patch clamping, confocal imaging, and flow cytometry. In the absence of barttin, only a small percentage of rCIC-K1 and hCIC-Kb channels are inserted into the plasma membrane. Coexpression of barttin enhances surface membrane insertion and furthermore modifies permeation and gating of CIC-K channels. hCIC-Kb channels are nonfunctional without barttin and require the coexpressed accessory subunit to become anion conducting. In contrast, rCIC-K1 channels are active without barttin, but at the cost of reduced unitary conductance as well as altered voltage dependence of activation. We mapped the separate functions of barttin to structural domains by a deletion analysis. Whereas the transmembrane core is necessary and sufficient to promote CIC-K channel exit from the endoplasmic reticulum, a short cytoplasmic segment following the second transmembrane helix modifies the unitary conductance. The entire cytoplasmic carboxyl terminus affects the open probability of CIC-K channels. The multiple functions of barttin might be necessary for a tight adjustment of epithelial Cl-conductances to ensure a precise regulation of body salt content and endocochlear potential.
机译：Barttin是在肾脏和内耳上皮细胞中表达的CIC型氯化物通道亚组的辅助亚基。在这项研究中，我们使用异源表达，膜片钳位，共聚焦成像和流式细胞术检测了Barttin对两种CIC-K通道同种型大鼠CIC-K1和人CIC-Kb的影响。在没有barttin的情况下，只有一小部分的rCIC-K1和hCIC-Kb通道被插入质膜。 Barttin的共表达增强了表面膜的插入，并进一步改善了CIC-K通道的渗透和门控。没有Barttin的hCIC-Kb通道是无功能的，需要共表达的附件亚基变为阴离子导电的。相比之下，rCIC-K1通道在没有barttin的情况下仍处于活动状态，但代价是单位电导降低以及激活的电压依赖性降低。我们通过缺失分析将巴特丁的单独功能映射到结构域。尽管跨膜核心对于促进CIC-K通道从内质网退出是必不可少的，但继第二个跨膜螺旋之后的短细胞质节段修饰了单位电导。整个细胞质羧基末端会影响CIC-K通道的开放可能性。严格调节上皮Cl的传导可能需要Barttin的多种功能，以确保精确调节体内盐含量和耳蜗内电位。

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