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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Identification of a protein complex that assembles lipopolysaccharide in the outer membrane of Escherichia coli
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Identification of a protein complex that assembles lipopolysaccharide in the outer membrane of Escherichia coli

机译：鉴定在大肠杆菌外膜中组装脂多糖的蛋白质复合物

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摘要

The outer membrane of most Gram-negative bacteria is made up of LIPS, and in nearly all bacteria that contain LIPS it is essential for the life of the organism. The lipid portion of this molecule, lipid A, also known as endotoxin, is a potent activator of the innate immune response. More than 50 genes are required to synthesize LIPS and assemble it at the cell surface. Enormous progress has been made in elucidating the structure and biosynthesis of LPS, but until recently the cellular components required for its transport from its site of synthesis in the inner membrane to its final cellular location at the cell surface remained elusive. Here we describe the identification of a protein complex that functions to assemble LPS at the surface of the cell. This complex contains two proteins: Imp, already identified as an essential outer-membrane protein implicated in LPS assembly; and another protein, RlpB, heretofore identified only as a rare lipoprotein. We show that RlpB is also essential for cell viability and that the Imp/RlpB complex is responsible for LIPS reaching the outer surface of the outer membrane.
机译：大多数革兰氏阴性细菌的外膜由LIPS组成，在几乎所有包含LIPS的细菌中，它对于生物体的生命都是必不可少的。该分子的脂质部分，脂质A，也称为内毒素，是先天免疫应答的有效激活剂。合成LIPS并将其组装在细胞表面需要50多个基因。在阐明LPS的结构和生物合成方面已取得了巨大进展，但是直到最近，从内膜的合成位点到细胞表面最终细胞定位所需的细胞成分仍然难以捉摸。在这里，我们描述了一种蛋白质复合物的鉴定，该蛋白质复合物的功能是在细胞表面组装LPS。该复合物包含两种蛋白质：Imp，已经被鉴定为与LPS组装有关的必需的外膜蛋白；迄今为止，另一种蛋白质RlpB仅被鉴定为稀有脂蛋白。我们显示RlpB对于细胞生存力也是必不可少的，并且Imp / RlpB复合物负责LIPS到达外膜的外表面。

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