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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Aptamers evolved from live cells as effective molecular probes for cancer study
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Aptamers evolved from live cells as effective molecular probes for cancer study

机译：适体从活细胞进化而来，是用于癌症研究的有效分子探针

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摘要

Using cell-based aptamer selection, we have developed a strategy to use the differences at the molecular level between any two types of cells for the identification of molecular signatures on the surface of targeted cells. A group of aptamers have been generated for the specific recognition of leukemia cells. The selected aptamers can bind to target cells with an equilibrium dissociation constant (K-d) in the nanomolar-to-picomolar range. The cell-based selection process is simple, fast, straightforward, and reproducible, and, most importantly, can be done without prior knowledge of target molecules. The selected aptamers can specifically recognize target leukemia cells mixed with normal human bone marrow aspirates and can also identify cancer cells closely related to the target cell line in real clinical specimens. The cell-based aptamer selection holds a great promise in developing specific molecular probes for cancer diagnosis and cancer biomarker discovery.
机译：使用基于细胞的适体选择，我们已经开发出一种策略，可以使用任意两种类型的细胞在分子水平上的差异来鉴定目标细胞表面的分子标记。已经产生了一组用于特异性识别白血病细胞的适体。选定的适体可以以纳摩尔至皮摩尔范围内的平衡解离常数（K-d）结合至靶细胞。基于细胞的选择过程简单，快速，简单且可重现，最重要的是，无需事先了解靶分子即可完成。所选的适体可以特异性识别与正常人骨髓穿刺细胞混合的靶白血病细胞，还可以在实际临床样本中鉴定与靶细胞系密切相关的癌细胞。基于细胞的适体选择在开发用于癌症诊断和癌症生物标志物发现的特定分子探针方面具有广阔的前景。

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