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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Manipulation of Arabidopsis fatty acid amide hydrolase expression modifies plant growth and sensitivity to N-acylethanolamines
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Manipulation of Arabidopsis fatty acid amide hydrolase expression modifies plant growth and sensitivity to N-acylethanolamines

机译：操纵拟南芥脂肪酸酰胺水解酶表达改变植物的生长和对N-酰基乙醇胺的敏感性

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摘要

In vertebrates, the endocannabinoid signaling pathway is an important lipid regulatory pathway that modulates a variety of physiological and behavioral processes. N-Acylethanolamines (NAEs) comprise a group of fatty acid derivatives that function within this pathway, and their signaling activity is terminated by an enzyme called fatty acid amide hydrolase (FAAH), which hydrolyzes NAEs to ethanolamine and their corresponding free fatty acids. Bioinformatic approaches led to the identification of plant homologues of FAAH that are capable of hydrolyzing NAEs in vitro. To better understand the role of NAEs in plants, we identified T-DNA knockouts to Arabidopsis FAAH (AtFAAH; At5g64440) and generated plants overexpressing AtFAAH. Here we show that seeds of AtFAAH knockouts had elevated levels of endogenous NAEs, and seedling growth was hypersensitive to exogenously applied NAE. On the other hand, seeds and seedlings of AtFAAH overexpressors had lower endogenous NAE content, and seedlings were less sensitive to exogenous NAE. Moreover, AtFAAH overexpressors displayed enhanced seedling growth and increased cell size. AtFAAH expression and FAAH catalytic activity increased during seed germination and seedling growth, consistent with the timing of NAE depletion during seedling establishment. Collectively, our results show that AtFAAH is one, but not the only, modulator of endogenous NAE levels in plants, and that NAE depletion likely participates in the regulation of plant growth.
机译：在脊椎动物中，内源性大麻素信号传导途径是重要的脂质调节途径，可调节多种生理和行为过程。 N-酰基乙醇胺（NAE）包含一组在该途径中起作用的脂肪酸衍生物，其信号传导活性被称为脂肪酸酰胺水解酶（FAAH）的酶终止，该酶将NAE水解为乙醇胺及其相应的游离脂肪酸。生物信息学方法导致鉴定出能够在体外水解NAE的FAAH植物同源物。为了更好地了解NAE在植物中的作用，我们鉴定了拟南芥FAAH（AtFAAH; At5g64440）的T-DNA敲除并生成了过表达AtFAAH的植物。在这里，我们显示AtFAAH基因敲除的种子具有较高水平的内源性NAEs，并且幼苗生长对外源应用的NAE高度敏感。另一方面，AtFAAH过表达子的种子和幼苗具有较低的内源性NAE含量，而幼苗对外源性NAE的敏感性较低。此外，AtFAAH过表达者表现出增强的幼苗生长和增加的细胞大小。在种子发芽和幼苗生长过程中，AtFAAH表达和FAAH催化活性增加，与幼苗建立过程中NAE消耗的时间一致。总的来说，我们的结果表明AtFAAH是植物中内源性NAE水平的一种但不是唯一的调节剂，并且NAE耗竭可能参与了植物生长的调节。

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