...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Monitoring the T cell response to genital tract infection
【24h】

Monitoring the T cell response to genital tract infection

机译：监测对生殖道感染的T细胞反应

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

To date, it has not been possible to study antigen-specific T cell responses during primary infection of the genital tract. The low frequency of pathogen-specific T cells in a naive mouse makes it difficult to monitor the initial events after antigen encounter. We developed a system to examine the response of pathogen-specific T cells in the genital mucosa after intrauterine infection. We identified the protective CD4(+) T cell antigen Cta1 from Chlamydia trachomatis and generated T cell receptor (TCR) transgenic (tg) mice with specificity for this protein. By transferring TCR tg T cells into naive animals, we determined that Chlamydia-specific T cells were activated and proliferated in the lymph nodes draining the genital tract after primary intrauterine infection. Activated T cells migrated into the genital mucosa and secreted IFN-gamma. The development of Chlamydia-specific TCR tg mice provides an approach for dissecting how pathogen-specific T cells function in the genital tract.
机译：迄今为止，尚不可能研究生殖道原发感染期间的抗原特异性T细胞应答。在幼稚的小鼠中，病原体特异性T细胞的频率较低，使得难以监测抗原遇到后的初始事件。我们开发了一个系统来检查子宫内感染后生殖器粘膜中病原体特异性T细胞的反应。我们从沙眼衣原体中鉴定出保护性CD4（+）T细胞抗原Cta1，并产生了对该蛋白具有特异性的T细胞受体（TCR）转基因（tg）小鼠。通过将TCR tg T细胞转移到幼稚动物中，我们确定衣原体特异性T细胞在原发性宫内感染后在引流生殖道的淋巴结中被激活并增殖。活化的T细胞迁移到生殖器粘膜并分泌IFN-γ。衣原体特异性TCR tg小鼠的发育为解剖病原体特异性T细胞在生殖道中的功能提供了一种方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号