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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Calbindin D-9k knockout mice are indistinguishable from wild-type mice in phenotype and serum calcium level
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Calbindin D-9k knockout mice are indistinguishable from wild-type mice in phenotype and serum calcium level

机译:钙结合蛋白D-9k基因敲除小鼠在表型和血清钙水平上与野生型小鼠没有区别

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摘要

Since the discovery of calbindin D9k, its role in intestinal calcium absorption has remained unsettled. Further, a wide distribution of calbindin D9k among tissues has argued for its biological importance. We discovered a frameshift deletion in the calbindin D9k gene in an ES cell line, E14.1, that originated from 129/OlaHsd mice. We produced mice with the mutant calbindin D9k gene by injecting the E14.1 ES cell subline into the C57BL/6 host blastocysts and proved that these mice lack calbindin D9k protein. Calbindin D9k knockout mice were indistinguishable from wild-type mice in phenotype, were able to reproduce, and had normal serum calcium levels. Thus, calbindin D9k is not required for viability, reproduction, or calcium homeostasis.
机译:自发现钙结合蛋白D9k以来,其在肠道钙吸收中的作用尚未确定。此外,钙结合蛋白D9k在组织中的广泛分布已证明其生物学重要性。我们在源自129 / OlaHsd小鼠的ES细胞系E14.1的calbindin D9k基因中发现了移码删除。通过将E14.1 ES细胞亚系注入C57BL / 6宿主胚泡中,我们生产了具有突变的calbindin D9k基因的小鼠,并证明这些小鼠缺乏calbindin D9k蛋白。钙结合蛋白D9k基因敲除小鼠在表型上与野生型小鼠没有区别,能够繁殖,并且血清钙水平正常。因此,不需要钙调蛋白D9k来维持生存能力,繁殖或维持钙稳态。

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