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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Selection and analysis of spontaneous reciprocal mitotic cross-overs in Saccharomyces cerevisiae
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Selection and analysis of spontaneous reciprocal mitotic cross-overs in Saccharomyces cerevisiae

机译：酿酒酵母自发有丝分裂的选择与分析

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摘要

We developed a system that allows the selection of the reciprocal products resulting from spontaneous mitotic cross-overs in the yeast Saccharomyces cerevisiae. A number of other types of genetic events, including chromosome loss, can be monitored with this system. For a 120-kb chromosome interval on chromosome V (CEN5-CAN1), the rate of mitotic cross-overs was 4 x 10(-5) per division, a rate approximate to 25,000-fold lower than the meiotic rate of crossovers. We found no suppression of mitotic cross-overs near the centromere of chromosome V, unlike the suppression observed for meiotic exchanges. The rate of reciprocal cross-overs was substantially (38-fold) elevated by treatment of cells with hydroxyurea, a drug that reduces nucleotide pools and slows DNA replication.
机译：我们开发了一种系统，该系统允许选择由酿酒酵母中的自发有丝分裂交叉产生的互惠产物。可以使用此系统监视许多其他类型的遗传事件，包括染色体丢失。对于V染色体（CEN5-CAN1）上一个120 kb的染色体区间，有丝分裂的通过率是每格4 x 10（-5），大约比减数分裂的通过率低25,000倍。我们发现没有抑制V染色体着丝粒附近的有丝分裂交叉，这与减数分裂交换所观察到的抑制不同。通过用羟基脲处理细胞，相互交换的速率大大提高（38倍），羟基脲是一种减少核苷酸库并减慢DNA复制的药物。

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