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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Simultaneous removal of sperm plasma membrane and acrosome before intracytoplasmic sperm injection improves oocyte activation/embryonic development
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Simultaneous removal of sperm plasma membrane and acrosome before intracytoplasmic sperm injection improves oocyte activation/embryonic development

机译：在胞浆内注射精子的同时去除精子质膜和顶体可改善卵母细胞的激活/胚胎发育

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摘要

Direct injection of a single spermatozoon into an oocyte (ICSI) can produce apparently normal offspring. Although the production of normal offspring by ICSI has been successful in mice and humans, it has been less successful in many other species. The reason for this is not clear, but could be, in part, due to inconsistent activation of oocytes because of delayed disintegration of sperm plasma membrane within oocytes and incorporation of the acrosome containing a spectrum of hydrolyzing enzymes. In the mouse, the removal of sperm plasma membrane and acrosome was not a prerequisite to produce offspring by ICSI, but it resulted in earlier onset of oocyte activation and better embryonic development. The best result was obtained when spermatozoa were demembranated individually immediately before ICSI by using lysolecithin, a hydrolysis product of membrane phospholipids.
机译：将单个精子直接注射到卵母细胞（ICSI）中可以产生明显正常的后代。尽管通过ICSI进行正常后代的生产已在小鼠和人类中获得成功，但在许多其他物种中却没有那么成功。其原因尚不清楚，但可能部分是由于卵母细胞内精子质膜的延迟崩解和掺入含有多种水解酶的顶体的掺入导致卵母细胞活化不一致所致。在小鼠中，去除精子质膜和顶体不是通过ICSI产生后代的先决条件，但它导致卵母细胞活化的早期发生和更好的胚胎发育。通过使用溶血卵磷脂（膜磷脂的水解产物）立即在ICSI之前将精子分别脱膜，可获得最佳结果。

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