...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Using cryo-EM to measure the dipole potential of a lipid membrane
【24h】

Using cryo-EM to measure the dipole potential of a lipid membrane

机译：使用cryo-EM测量脂质膜的偶极电位

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The dipole potential of a lipid bilayer membrane accounts for its much larger permeability to anions than cations and affects the conformation and function of membrane proteins. The absolute value of the dipole potential has been very difficult to measure, although its value has been estimated to range from 200 to 1,000 mV from ion translocation rates, the surface potential of lipid monolayers, and molecular dynamics calculations. Here, a point charge probe method was used to investigate the dipole potentials of both ester and ether lipid membranes. The interactions between electrons and lipid molecules were recorded by phase-contrast imaging using cryo-EM. The magnitude and the profile of the dipole potential along the bilayer normal were obtained by subtracting the contribution of the atomic potential from the cryo-EM image intensity. The peak dipole potential was estimated to be 510 and 260 mV for diphytanoylphosphatidylcholine and diphiytanylphosphatidylcholine, respectively.
机译：脂质双层膜的偶极电位是其对阴离子的渗透性比阳离子大的原因，并影响膜蛋白的构象和功能。尽管根据离子迁移速率，脂质单分子层的表面电势和分子动力学计算，其偶极电势的绝对值估计为200至1,000 mV，但很难测量。在这里，使用点电荷探针法研究酯和醚脂质膜的偶极电势。电子和脂质分子之间的相互作用是通过使用cryo-EM的相差成像记录的。通过从低温EM图像强度中减去原子电势的贡献，可以得到沿着双层法线的偶极电势的大小和轮廓。对于二植烷酰基磷脂酰胆碱和二phitantanyl磷脂酰胆碱，峰值偶极电势估计分别为510和260 mV。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号