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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Optical tracking of organically modified silica nanoparticles as DNA carriers: a nonviral, nanomedicine approach for gene delivery.
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Optical tracking of organically modified silica nanoparticles as DNA carriers: a nonviral, nanomedicine approach for gene delivery.

机译：有机修饰的二氧化硅纳米粒子作为DNA载体的光学跟踪：一种非病毒，纳米药物的基因传递方法。

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This article reports a multidisciplinary approach to produce fluorescently labeled organically modified silica nanoparticles as a nonviral vector for gene delivery and biophotonics methods to optically monitor intracellular trafficking and gene transfection. Highly monodispersed, stable aqueous suspensions of organically modified silica nanoparticles, encapsulating fluorescent dyes and surface functionalized by cationic-amino groups, are produced by micellar nanochemistry. Gel-electrophoresis studies reveal that the particles efficiently complex with DNA and protect it from enzymatic digestion of DNase 1. The electrostatic binding of DNA onto the surface of the nanoparticles, due to positively charged amino groups, is also shown by intercalating an appropriate dye into the DNA and observing the Forster (fluorescence) resonance energy transfer between the dye (energy donor) intercalated in DNA on the surface of nanoparticles and a second dye (energy acceptor) inside the nanoparticles. Imaging by fluorescence confocal microscopy shows that cells efficiently take up the nanoparticles in vitro in the cytoplasm, and the nanoparticles deliver DNA to the nucleus. The use of plasmid encoding enhanced GFP allowed us to demonstrate the process of gene transfection in cultured cells. Our work shows that the nanomedicine approach, with nanoparticles acting as a drug-delivery platform combining multiple optical and other types of probes, provides a promising direction for targeted therapy with enhanced efficacy as well as for real-time monitoring of drug action.
机译：本文报道了一种多学科的方法来生产荧光标记的有机修饰的二氧化硅纳米颗粒，将其作为非病毒载体用于基因传递和生物光子学方法，以光学方式监测细胞内运输和基因转染。通过胶束纳米化学制备了有机改性的二氧化硅纳米粒子的高度单分散，稳定的水悬浮液，其封装了荧光染料并被阳离子氨基官能化。凝胶电泳研究表明，该颗粒可与DNA高效复合，并保护其免受酶DNase 1的酶解。由于带正电荷的氨基，DNA与纳米颗粒表面的静电结合也可通过将适当的染料嵌入DNA并观察纳米粒子表面DNA中插入的染料（能量供体）和纳米粒子内部的第二种染料（能量受体）之间的Forster（荧光）共振能量转移。通过荧光共聚焦显微镜成像显示，细胞在体外有效地吸收了细胞质中的纳米粒子，并且纳米粒子将DNA传递至细胞核。使用编码增强的GFP的质粒使我们能够证明在培养细胞中基因转染的过程。我们的工作表明，纳米药物结合纳米粒子作为药物输送平台，将多种光学探针和其他类型的探针结合在一起，为具有增强功效的靶向治疗以及实时监测药物作用提供了有希望的方向。

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