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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Peptide signaling during terminal differentiation of Dictyostelium
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Peptide signaling during terminal differentiation of Dictyostelium

机译：盘基网柄菌终末分化过程中的肽信号传导

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摘要

A wide variety of mechanisms have evolved for intercellular communication in metazoans, but some of the signaling molecules were already used in their predecessors. The social amoeba, Dictyostelium discoideum, is known to use peptides to trigger sporu-lation within fruiting bodies, but their sequences have not been defined. We found that the peptide signal spore differentiation factor 2 (SDF-2) is processed from acyl-CoA binding protein, AcbA. The mammalian homolog of AcbA is processed to diazepam binding inhibitor that binds to the GABA_A receptor in the brain and to peripheral 1,4 benzodiazepine receptors. Although Dictyostelium has neither GABAA nor peripheral-type benzodiazepine receptors, we find that both a diazepam binding inhibitor peptide and diazepam (Valium) can mimic SDF-2 in a Dictyostelium bioassay. Mutants lacking AcbA sporufate well only when developed in chimeras with WT cells. Using a yeast system we show that ligand binding to the SDF-2 receptor histidine kinase, DhkA, inhibits phosphorelay, which can account for its ability to induce rapid sporulation.
机译：在后生动物中已经发展出多种机制来进行细胞间通讯，但是一些信号分子已经在其前身中使用。已知社交变形虫，盘基网柄菌（Dictyostelium discoideum）使用肽来触发子实体内的孢子形成，但尚未定义它们的序列。我们发现，肽信号孢子分化因子2（SDF-2）是由酰基辅酶A结合蛋白AcbA加工而成的。 AcbA的哺乳动物同源物被加工成地西epa结合抑制剂，该抑制剂与大脑中的GABA_A受体和周围的1,4苯并二氮杂receptor受体结合。尽管Dictyostelium既不具有GABAA也不具有外周型苯并二氮杂receptor受体，但是我们发现Diazyom结合抑制剂肽和diazepam（Valium）均可在Dictyostelium生物测定中模拟SDF-2。缺乏AcbA的突变体只有在嵌合体与WT细胞一起发育时才能充分繁殖。使用酵母系统，我们显示与SDF-2受体组氨酸激酶DhkA结合的配体抑制了磷灰质，这可以解释其诱导快速孢子形成的能力。

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