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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >ExsE, a secreted regulator of type Ⅲ secretion genes in Pseudomonas aeruginosa
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ExsE, a secreted regulator of type Ⅲ secretion genes in Pseudomonas aeruginosa

机译：ExsE，铜绿假单胞菌Ⅲ型分泌基因的分泌调控因子

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摘要

Type Ⅲ secretion systems are toxin delivery systems that are present in a large number of pathogens. A hallmark of all type Ⅲ secretion systems studied to date is that expression of one or more of their components is induced upon cell contact. It has been proposed that this induction is controlled by a negative regulator that is itself secreted by means of the type Ⅲ secretion machinery. Although candidate proteins for this negative regulator have been proposed in a number of systems, for the most part, a direct demonstration of their role in regulation is lacking. Here, we report the discovery of ExsE, a negative regulator of type Ⅲ secretion gene expression in Pseudomonas aeruginosa. Deletion of exsE deregulates expression of the type Ⅲ secretion genes. We provide evidence that ExsE is itself secreted by means of the type Ⅲ secretion machinery and physically interacts with ExsC, a positive regulator of the type Ⅲ secretion regulon. Taken together, these data demonstrate that ExsE is the secreted negative regulator that couples triggering of the type Ⅲ secretion machinery to induction of the type Ⅲ secretion genes.
机译：Ⅲ型分泌系统是毒素输送系统，存在于大量病原体中。迄今为止研究的所有Ⅲ型分泌系统的标志是，细胞接触都会诱导其一种或多种成分的表达。已经提出这种诱导是由负调节剂控制的，该负调节剂本身是通过Ⅲ型分泌机制分泌的。尽管已经在许多系统中提出了这种负调节剂的候选蛋白，但在大多数情况下，仍缺乏直接证明其在调节中作用的证据。在这里，我们报告发现ExsE，铜绿假单胞菌中Ⅲ型分泌基因表达的负调节剂。 exsE的缺失会破坏Ⅲ型分泌基因的表达。我们提供的证据表明，ExsE本身是通过Ⅲ型分泌机制分泌的，并且与ExsC（Ⅲ型分泌调节剂的阳性调节剂）发生物理相互作用。综上所述，这些数据表明，ExsE是分泌的负调节剂，其将Ⅲ型分泌机制的触发与Ⅲ型分泌基因的诱导耦合在一起。

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