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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Spatial location and requirements for the assembly of the Agrobacterium tumefaciens type Ⅳ secretion apparatus
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Spatial location and requirements for the assembly of the Agrobacterium tumefaciens type Ⅳ secretion apparatus

机译：根癌农杆菌Ⅳ型分泌器具组装的空间位置和要求

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摘要

Type Ⅳ secretion is used by pathogenic microorganisms to transfer effector macromolecules to eukaryotic target cells. The VirB/D4 apparatus of Agrobacterium tumefaciens transfers DNA and proteins to plant cells. We postulated that the cell pole is the site of assembly of the A. tumefaciens type Ⅳ apparatus. Using immuno-fluorescence microscopy, we now demonstrate that 10 of the VirB proteins localized primarily to one cell pole and a macromolecular VirB complex is assembled at the pole. Neither the assembly of the complex nor polar localization of a VirB protein requires ATP utilization by the VirB ATPases. The requirement of other VirB proteins for the polar localization of at least six VirB proteins indicates an essential role of protein-protein interaction in polar targeting. Four proteins (VirB3, VirB4, VirB8, and VirB11) could target themselves to a cell pole independent of a VirB protein. We provide evidence that VirB6-VirB10 are the structural components of the type Ⅳ apparatus. Using strains that express defined subsets of the virB genes, we demonstrate that VirB7-VirB10 are the minimum components sufficient for the assembly of a polar VirB complex. VirB6 associates with this complex to form the type Ⅳ secretion apparatus. VirB8 functions as the assembly factor and targets the apparatus to the cell pole.
机译：病原微生物利用Ⅳ型分泌物将效应大分子转移到真核靶细胞中。根癌农杆菌的VirB / D4装置将DNA和蛋白质转移至植物细胞。我们推测细胞极是根癌农杆菌Ⅳ型装置的组装位点。使用免疫荧光显微镜，我们现在证明10个VirB蛋白主要定位于一个细胞极，大分子VirB复合物在该极组装。 VirB蛋白的复合物装配和极性定位都不要求VirB ATPase对ATP的利用。对于至少六个VirB蛋白的极性定位，还需要其他VirB蛋白，这表明蛋白-蛋白相互作用在极性靶向中至关重要。四种蛋白质（VirB3，VirB4，VirB8和VirB11）可以将自身靶向至独立于VirB蛋白的细胞极。我们提供的证据表明VirB6-VirB10是Ⅳ型仪器的结构组件。使用表达virB基因的定义子集的菌株，我们证明VirB7-VirB10是足以组装极性VirB复合物的最小成分。 VirB6与该复合物结合形成Ⅳ型分泌设备。 VirB8充当组装因子，并将设备对准电池极。

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