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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The x-ray crystal structure of lysine-2,3-aminomutase from Clostridium subterminale
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The x-ray crystal structure of lysine-2,3-aminomutase from Clostridium subterminale

机译：梭状芽胞杆菌末端的赖氨酸-2,3-氨基变位酶的X射线晶体结构

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摘要

The x-ray crystal structure of the pyrid oxa 1-5'-phosphate (PLP), S-adenosyl-L-methionine (SAM), and [4Fe-4S]-dependent lysine-2,3-aminomutase (LAM) of Clostridium subterminale has been solved to 2.1-angstrom resolution by single-wavelength anomalous dispersion methods on a L-selenomethionine-substituted complex of LAM with [4Fe-4S](2+), PLP, SAM, and L-alpha-lysine, a very close analog of the active Michaelis complex. The unit cell contains a dimer of hydrogen-bonded, domain-swapped dimers, the subunits of which adopt a fold that contains all three cofactors in a central channel defined by six beta/alpha structural units. Zinc coordination links the domain-swapped dimers. In each subunit, the solvent face of the channel is occluded by an N-terminal helical domain, with the opposite end of the channel packed against the domain-swapped subunit. Hydrogen-bonded ionic contacts hold the external aldimine of PLP and L-alpha-lysine in position for abstraction of the 3-pro-R hydrogen of lysine by C5' of SAM. The structure of the SAM/[4Fe4S] complex confirms and extends conclusions from spectroscopic studies of LAM and shows selenium in Se-adenosyl-L-selenomethionine poised to ligate the unique iron in the [4Fe-4S] cluster upon electron transfer and radical formation. The chain fold in the central domain is in part analogous to other radical-SAM enzymes.
机译：X射线晶体结构的吡啶氧1-5'-磷酸（PLP），S-腺苷-L-蛋氨酸（SAM）和[4Fe-4S]依赖性赖氨酸-2,3-氨基变位酶（LAM）已通过单波长异常分散方法在LAM的L-硒代蛋氨酸取代的复合物与[4Fe-4S]（2 +），PLP，SAM和L-α-赖氨酸（一种与活跃的Michaelis复合体非常相似。晶胞包含氢键键合的，域交换的二聚体的二聚体，其亚基采用一个折叠，该折叠包含由六个β/α结构单元定义的中央通道中的所有三个辅因子。锌配位链接了域交换的二聚体。在每个亚基中，通道的溶剂面被N末端螺旋结构域阻塞，通道的另一端紧靠结构域交换的亚基。氢键离子触点将PLP和L-α-赖氨酸的外部醛亚胺固定在适当位置，以通过SAM的C5'提取赖氨酸的3-pro-R氢。 SAM / [4Fe4S]配合物的结构证实并扩展了LAM光谱研究的结论，并显示了Se-腺苷-L-硒代蛋氨酸中的硒在电子转移和自由基形成后准备结扎[4Fe-4S]簇中的独特铁。 。中央结构域中的链折叠部分类似于其他自由基-SAM酶。

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