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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Histone acetylation affects expression of cellular patterning genes in the Arabidopsis root epidermis
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Histone acetylation affects expression of cellular patterning genes in the Arabidopsis root epidermis

机译：组蛋白乙酰化影响拟南芥根表皮中细胞模式基因的表达

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摘要

The Arabidopsis root has a unique cellular pattern in its single-layered epidermis. Cells residing over the intercellular spaces between underlying cortical cells (H position) differentiate into hair cells, whereas those directly over cortical cells (N position) differentiate into non-hair cells. Recent studies have revealed that this cellular pattern is determined by interactions of six patterning genes CPC, ETC, GL2, GL3/EGL3, TTG, and WER, and that the position-dependent expression of the CPC, GL2, and WER genes is essential for their appropriate interactions. However, little is known about how the expressions of the pattern genes are determined. Here we show that trichostatin A (TSA) treatment of germinating Arabidopsis seedlings alters the cellular pattern of the root epidermis to induce hair cell development at nonhair positions. The effects of TSA treatment are rapid, reversible, concentration-dependent, and position-independent. TSA inhibition of histone deacetylase activity results in hyperacetylation of the core histones H3 and H4, and alters the expression levels and cell specific expression of the patterning genes CPC, GL2 and WER. Analysis of histone deacetylase mutant cellular patterning further verified the participation of histone acetylation in cellular patterning, and revealed that HDA18 is a key component in the regulatory machinery of the Arabidopsis root epidermis. We propose a working model to suggest that histone acetylation may function in mediating a positional cue to direct expression of the patterning genes in the root epidermal cells.
机译：拟南芥的根在其单层表皮中具有独特的细胞模式。位于下层皮质细胞（H位置）之间的细胞间空间的细胞分化为毛细胞，而直接位于皮质细胞（N位置）之上的细胞则分化为非毛细胞。最近的研究表明，这种细胞模式是由六个模式基因CPC，ETC，GL2，GL3 / EGL3，TTG和WER的相互作用决定的，而CPC，GL2和WER基因的位置依赖性表达对于他们适当的互动。但是，关于如何确定模式基因的表达知之甚少。在这里，我们显示了曲古抑菌素A（TSA）对发芽拟南芥幼苗的处理会改变根表皮的细胞模式，从而在非毛发位置诱导毛细胞发育。 TSA治疗的作用是快速，可逆，浓度依赖性和位置依赖性。 TSA抑制组蛋白脱乙酰基酶活性导致核心组蛋白H3和H4过度乙酰化，并改变了模式基因CPC，GL2和WER的表达水平和细胞特异性表达。对组蛋白脱乙酰基酶突变体细胞模式的分析进一步证实了组蛋白乙酰化在细胞模式中的参与，并揭示了HDA18是拟南芥根表皮调节机制中的关键成分。我们提出了一个工作模型，以表明组蛋白乙酰化可能在介导位置指示基因在表皮根细胞中直接表达的功能中起作用。

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