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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Dynamic transport of SNARE proteins in the Golgi apparatus.
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Dynamic transport of SNARE proteins in the Golgi apparatus.

机译：SNARE蛋白在高尔基体中的动态转运。

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摘要

Localization of a membrane protein in a subcellular compartment can be achieved by its retention in the compartment or by its continuous transport toward this compartment. Previous results have suggested that specific enzymes are localized in the Golgi apparatus at least in part by selective retention and exclusion from transport vesicles. However, the function of some Golgi SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins is not compatible with their exclusion from transport vesicles. To help understand the mechanism accounting for the localization of SNARE proteins in the Golgi apparatus, we analyzed their lateral distribution in the Golgi cisternae and their incorporation into transport vesicles. According to our results, all SNARE proteins are efficiently incorporated into transport vesicles, indicating that the localization of SNARE proteins in the Golgi apparatus is not based on a static retention mechanism. Detailed analysis suggested that incorporation intotransport vesicles was more efficient for SNARE proteins restricted to the cis face of the Golgi as compared with SNAREs present at the trans face. Furthermore, overexpression of a cis-Golgi SNARE protein altered concomitantly its incorporation in transport vesicles and its intra-Golgi localization. These observations suggest that, contrary to resident Golgi enzymes, SNARE proteins are localized in the Golgi apparatus as the result of a dynamic transport equilibrium.
机译：膜蛋白在亚细胞区室中的定位可以通过其在区室中的保留或向该区室的连续运输来实现。先前的结果表明，特定的酶至少部分地通过选择性保留和从运输小泡中排除而定位在高尔基体中。但是，某些高尔基SNARE（可溶性N-乙基马来酰亚胺敏感因子附着蛋白受体）蛋白的功能与其从运输小泡中排除的功能不兼容。为了帮助理解解释SNARE蛋白在高尔基体中定位的机理，我们分析了它们在高尔基池中的侧向分布及其在运输囊泡中的结合。根据我们的结果，所有SNARE蛋白都有效地整合到转运囊泡中，这表明SNARE蛋白在高尔基体中的定位不是基于静态保留机制。详细的分析表明，与存在于反式表面的SNARE相比，掺入运输小泡对限制在高尔基体顺式表面的SNARE蛋白质更有效。此外，顺式-高尔基SNARE蛋白的过表达随之改变了其在运输小泡中的掺入及其在高尔基体内的定位。这些观察结果表明，与驻留的高尔基酶相反，作为动态转运平衡的结果，SNARE蛋白位于高尔基体中。

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