Solution structure of the Set2-Rpb1 interacting domain of human Set2 and its interaction with the hyperphosphorylated C-terminal domain of Rpb1.

Li M; Phatnani HP; Guan Z; Sage H; Greenleaf AL; Zhou P

首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Solution structure of the Set2-Rpb1 interacting domain of human Set2 and its interaction with the hyperphosphorylated C-terminal domain of Rpb1.

【24h】

Solution structure of the Set2-Rpb1 interacting domain of human Set2 and its interaction with the hyperphosphorylated C-terminal domain of Rpb1.

机译：人Set2的Set2-Rpb1相互作用域的溶液结构及其与Rpb1的超磷酸化C末端域的相互作用。

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

The phosphorylation state of the C-terminal repeat domain (CTD) of the largest subunit of RNA polymerase II changes as polymerase transcribes a gene, and the distinct forms of the phospho-CTD (PCTD) recruit different nuclear factors to elongating polymerase. The Set2 histone methyltransferase from yeast was recently shown to bind the PCTD of elongating RNA polymerase II by means of a novel domain termed the Set2-Rpb1 interacting (SRI) domain. Here, we report the solution structure of the SRI domain in human Set2 (hSRI domain), which adopts a left-turned three-helix bundle distinctly different from other structurally characterized PCTD-interacting domains. NMR titration experiments mapped the binding surface of the hSRI domain to helices 1 and 2, and Biacore binding studies showed that the domain binds preferably to [Ser-2 + Ser-5]-phosphorylated CTD peptides containing two or more heptad repeats. Point-mutagenesis studies identified five residues critical for PCTD binding. In view of the differential effects of these point mutations on binding to different CTD phosphopeptides, we propose a model for the hSRI domain interaction with the PCTD.

机译：RNA聚合酶II的最大亚基的C末端重复结构域（CTD）的磷酸化状态随聚合酶转录基因而变化，并且磷酸化CTD（PCTD）的不同形式募集不同的核因子来延长聚合酶。最近显示，来自酵母的Set2组蛋白甲基转移酶通过称为Set2-Rpb1相互作用（SRI）域的新型域与延长RNA聚合酶II的PCTD结合。在这里，我们报告人类Set2（hSRI域）中的SRI域的解决方案结构，该结构采用了一个左转的三螺旋束，该束与其他结构表征的PCTD相互作用域明显不同。 NMR滴定实验将hSRI结构域与螺旋1和2的结合表面作图，Biacore结合研究表明该结构域优选与含有两个或多个七肽重复序列的[Ser-2 + Ser-5]磷酸化CTD肽结合。点诱变研究确定了对PCTD结合至关重要的五个残基。考虑到这些点突变对与不同CTD磷酸肽结合的不同影响，我们提出了hSRI结构域与PCTD相互作用的模型。

著录项

来源
《Proceedings of the National Academy of Sciences of the United States of America》 |2005年第49期|P.17636-17641|共6页
作者
Li M; Phatnani HP; Guan Z; Sage H; Greenleaf AL; Zhou P;
展开▼
作者单位

Department of Biochemistry, Duke University Medical Center, Research Drive, Durham, NC 27710, USA.;

展开▼
收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类基础医学;
关键词
binding; structure; Carbon ion; Solutions; Human; 溶液;

机译：结合;结构;碳离子;溶液;人;溶液;

相似文献

外文文献
中文文献
专利

1. Solution NMR structure of the ARID domain of human AT-rich interactive domain-containing protein 3A: a human cancer protein interaction network target. [J] . Liu G, Huang YJ, Xiao R, Proteins: Structure, Function, and Genetics . 2010,第9期

机译：富含人AT相互作用域的蛋白质3A的ARID域的溶液NMR结构：人类癌症蛋白相互作用网络的目标。
2. Solution structure of the C-terminal X domain of the measles virus phosphoprotein and interaction with the intrinsically disordered C-terminal domain of the nucleoprotein. [J] . Gely S, Lowry DF, Bernard C, Journal of molecular recognition: JMR . 2010,第5期

机译：麻疹病毒磷蛋白C末端X结构域的溶液结构以及与核蛋白固有无序的C末端结构域的相互作用。
3. Solution structure of the PDZ2 domain from human phosphatase hPTP1E and its interactions with C-terminal peptides from the Fas receptor. [J] . Kozlov G, Gehring K, Ekiel I Biochemistry . 2000,第10期

机译：人类磷酸酶hPTP1E的PDZ2域的溶液结构及其与Fas受体C端肽的相互作用。
4. Solution Structure of Homeobox Transcription Factor Hex C-terminal Domain of Negatively Charged Activating Function [C] . Shunsuke Meshitsuka, Yuki Horie, Kazuya Takahashi, Advances in biomedical research . 2010

机译：带负电激活功能的同源异型盒转录因子十六进制C末端域的解决方案结构
5. Crystal structures of the Fanconi anemia proteins: Structure of the interstrand cross-linking repair protein Fanconi anemia protein I (FANCI); structure of the human FANCF C-terminal domain; reconstitution and crystallilzation of the sub-complexes in the Fanconi anemia core complex. [D] . Xu, Guozhou. 2009

机译：Fanconi贫血蛋白的晶体结构：链间交联修复蛋白Fanconi贫血蛋白I（FANCI）的结构；人FANCF C末端结构域的结构； Fanconi贫血核心复合物中亚复合物的重组和结晶。
6. Solution structure of the Set2–Rpb1 interacting domain of human Set2 and its interaction with the hyperphosphorylated C-terminal domain of Rpb1 [O] . Ming Li, Hemali P. Phatnani, Ziqiang Guan, 2005

机译：人Set2的Set2-Rpb1相互作用域的溶液结构及其与Rpb1的超磷酸化C末端域的相互作用
7. Solution structure of the Set2–Rpb1 interacting domain of human Set2 and its interaction with the hyperphosphorylated C-terminal domain of Rpb1 [O] . Li, Ming, Phatnani, Hemali P., Guan, Ziqiang, 2005

机译：人Set2的Set2-Rpb1相互作用域的溶液结构及其与Rpb1的超磷酸化C末端域的相互作用

Solution structure of the Set2-Rpb1 interacting domain of human Set2 and its interaction with the hyperphosphorylated C-terminal domain of Rpb1.

摘要

著录项

相似文献

相关主题

期刊订阅