首页> 外文会议>Advances in biomedical research >Solution Structure of Homeobox Transcription Factor Hex C-terminal Domain of Negatively Charged Activating Function

【24h】

Solution Structure of Homeobox Transcription Factor Hex C-terminal Domain of Negatively Charged Activating Function

机译：带负电激活功能的同源异型盒转录因子十六进制C末端域的解决方案结构

获取原文

获取原文并翻译 | 示例

页面导航

摘要
著录项
相似文献
相关主题

摘要

Transcription factor Hex (haematopoietically expressed homeobox) is a homeobox protein that regulates differentiation and development of liver and monocyte. Hex consists of three functional domains, N-terminal proline rich domain, homeodomain and acidic C terminal domain. Other than DNA binding, the function of Hex is to repress and activate transcription of target genes using multiple mechanism. However, the structure and function of Hex-C terminal domain is not yet elucidated. Hence, the present structural studies were carried out by NMR spectroscopy in order to understand the function of the Hex-C domain. We deteremined the NMR assignment of backbone and side chains, and obtained 3D structure of Hex-C domain in the presence of GST tag. This is the first paper to present the structure of a protein with GST tag by NMR.

机译：转录因子Hex（造血表达的同源盒）是一种同源盒蛋白，可调节肝脏和单核细胞的分化和发育。十六进制由三个功能域组成：N末端富含脯氨酸的域，同源域和酸性C末端域。除DNA结合外，Hex的功能是使用多种机制抑制和激活靶基因的转录。但是，Hex-C末端域的结构和功能尚未阐明。因此，目前的结构研究是通过NMR光谱进行的，以了解Hex-C结构域的功能。我们确定了骨架和侧链的NMR分配，并在存在GST标签的情况下获得了Hex-C结构域的3D结构。这是第一篇通过NMR展示具有GST标签的蛋白质结构的论文。

著录项

来源
《Advances in biomedical research》|2010年|p.301-306|共6页
会议地点 Cambridge(GB);Cambridge(GB);Cambridge(GB);Cambridge(GB);Cambridge(GB);Cambridge(GB)
作者
Shunsuke Meshitsuka; Yuki Horie; Kazuya Takahashi; Ojeiru F. Ezomo; Mohammed S. Mustak; et al;
展开▼
作者单位

Institute of Regenerative Medicine and Biofunction Tottori University Graduate School of Medical Science, 68 Nishimachi Yonago Tottori 683-8503 JAPAN;

Institute of Regenerative Medicine and Biofunction Tottori University Graduate School of Medical Science, 68 Nishimachi Yonago Tottori 683-8503 JAPAN;

Institute of Regenerative Medicine and Biofunction Tottori University Graduate School of Medical Science, 68 Nishimachi Yonago Tottori 683-8503 JAPAN;

Institute of Regenerative Medicine and Biofunction Tottori University Graduate School of Medical Science, 68 Nishimachi Yonago Tottori 683-8503 JAPAN;

Institute of Regenerative Medicine and Biofunction Tottori University Graduate School of Medical Science, 68 Nishimachi Yonago Tottori 683-8503 JAPAN;

et al;

展开▼
会议组织
原文格式 PDF
正文语种 eng
中图分类生物医学工程;
关键词
transcription factor; NMR; Hex; GST; protein tag; domain structure;

机译：转录因子NMR;十六进制消费税;蛋白质标签领域结构;

相似文献

外文文献
中文文献
专利

1. Solution Structure of the C-Terminal Transcriptional Activator Domain of FixJ from Sinorhizobium meliloti and Its Recognition of the fixK Promoter [J] . Kaori Kurashima-Ito, Yuichi Kasai, Kaito Hosono, Biochemistry . 2005,第45期

机译：来自Sinorhizobium Meliloti的FixJ的C末端转录激活域的溶液结构及其识别夹克启动子
2. Role of trehalose and heat in the structure of the C-terminal activation domain of the heat shock transcription factor. [J] . Bulman AL, Nelson HC Proteins: Structure, Function, and Genetics . 2005,第4期

机译：海藻糖和热在热休克转录因子的C末端活化域结构中的作用。
3. High precision solution structure of the C-terminal KH domain of heterogeneous nuclear ribonucleoprotein K, a c-myc transcription factor. [J] . Baber JL, Libutti D, Levens D, Journal of Molecular Biology . 1999,第4期

机译：异质核糖核蛋白K（c-myc转录因子）C端KH结构域的高精度溶液结构。
4. Solution Structure of Homeobox Transcription Factor Hex C-terminal Domain of Negatively Charged Activating Function [C] . Shunsuke Meshitsuka, Yuki Horie, Kazuya Takahashi, WSEAS International Conference on Mathematical Biology and Ecology . 2010

机译：Homeobox转录因子六角形六端六端六端六角形域的溶液结构是带负电荷的激活功能
5. A mutational and functional analysis of C-terminal sequences of human transcription factor REL: Their role in cellular transformation and transcriptional activation. [D] . Starczynowski, Daniel T. 2006

机译：人类转录因子REL C末端序列的突变和功能分析：它们在细胞转化和转录激活中的作用。
6. NMR solution structure and function of the C-terminal domain of eukaryotic class 1 polypeptide chain release factor [O] . Alexey B Mantsyzov, Elena V Ivanova, Berry Birdsall, -1

机译：真核生物1类多肽链释放因子C端结构域的NMR溶液结构和功能
7. Solution Structure of the C-Terminal Transcriptional Activator Domain of FixJ from Sinorhizobium meliloti and Its Recognition of the fixK Promoter [O] . -1

机译：来自Sinorhizobium Meliloti的FixJ的C-末端转录域的溶液结构及其对FixK启动子的识别

Solution Structure of Homeobox Transcription Factor Hex C-terminal Domain of Negatively Charged Activating Function

摘要

著录项

相似文献

相关主题

期刊订阅