...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The planarian Schmidtea mediterranea as a model for epigenetic germ cell specification: analysis of ESTs from the hermaphroditic strain.
【24h】

The planarian Schmidtea mediterranea as a model for epigenetic germ cell specification: analysis of ESTs from the hermaphroditic strain.

机译：planar虫Schmidtea mediterranea作为表观遗传生殖细胞规范的模型：来自雌雄同体菌株的EST分析。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Freshwater planarians have prodigious regenerative abilities that enable them to form complete organisms from tiny body fragments. This plasticity is also exhibited by the planarian germ cell lineage. Unlike many model organisms in which germ cells are specified by localized determinants, planarian germ cells appear to be specified epigenetically, arising postembryonically from stem cells. The planarian Schmidtea mediterranea is well suited for investigating the mechanisms underlying epigenetic germ cell specification. Two strains of S. mediterranea exist: a hermaphroditic strain that reproduces sexually and an asexual strain that reproduces by means of transverse fission. To date, expressed sequence tags (ESTs) have been generated only from the asexual strain. To develop molecular reagents for studying epigenetic germ cell specification, we have sequenced 27,161 ESTs from two developmental stages of the hermaphroditic strain of S. mediterranea; this collection of ESTs represents approximately 10,000 unique transcripts. blast analysis of the assembled ESTs showed that 66% share similarity to sequences in public databases. We annotated the assembled ESTs using Gene Ontology terms as well as conserved protein domains and organized them in a relational database. To validate experimentally the Gene Ontology annotations, we used whole-mount in situ hybridization to examine the expression patterns of transcripts assigned to the biological process "reproduction." Of the 53 genes in this category, 87% were expressed in the reproductive organs. In addition to its utility for studying germ cell development, this EST collection will be an important resource for annotating the planarian genome and studying this animal's amazing regenerative abilities.
机译：淡水浮游动物具有惊人的再生能力，使他们能够从微小的身体碎片中形成完整的生物。平面虫生殖细胞谱系也表现出这种可塑性。与许多模型生物通过定位决定簇指定生殖细胞不同，涡虫生殖细胞似乎是表观遗传学指定的，是胚胎后起源于干细胞的。平面虫Schmidtea mediterranea非常适合用于研究表观遗传生殖细胞规格的基础机制。存在两种S. Mediterraterranea菌株：一种通过性繁殖的雌雄同体的菌株，以及通过横向裂变繁殖的无性菌株。迄今为止，仅由无性菌株产生表达的序列标签（EST）。为了开发用于研究表观遗传生殖细胞规格的分子试剂，我们已经从S. mediterranea雌雄同体菌株的两个发育阶段测序了27,161个EST。 EST的这个集合代表大约10,000个唯一的成绩单。组装的EST的爆炸分析表明，有66％的人与公共数据库中的序列具有相似性。我们使用基因本体论术语以及保守的蛋白质结构域对组装的EST进行了注释，并将其组织在关系数据库中。为了通过实验验证基因本体论注释，我们使用了整装原位杂交技术来检查分配给生物过程“复制”的转录本的表达模式。在该类别的53个基因中，有87％在生殖器官中表达。除了可用于研究生殖细胞的发育外，该EST收集品还将成为注释涡虫基因组和研究该动物惊人的再生能力的重要资源。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号