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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The opportunistic pathogen Pseudomonas aeruginosa carries a secretable arachidonate 15-lipoxygenase
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The opportunistic pathogen Pseudomonas aeruginosa carries a secretable arachidonate 15-lipoxygenase

机译：机会病原体铜绿假单胞菌携带一种分泌型花生四烯酸酯15-脂加氧酶

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摘要

In mammals, lipoxygenases play key roles in inflammation by initiating the transformation of arachidonic acid into potent bio-active lipid mediators such as leukotrienes and lipoxins. In general, most bacteria are believed to lack lipoxygenases and their poly-unsaturated fatty acid substrates. It is therefore of interest that an ORF (PA1169) with high homology to eukaryotic lipoxygenases was discovered by analysis of the whole-genome sequence of the opportunistic bacterial pathogen Pseudomonas aeruginosa. Using TLC and liquid chromatography-UV-tandem mass spectrometry (LC-UV-MS-MS), we demonstrate that PA1169 encodes a bacterial lipoxygenase (LoxA) that converts arachidonic acid into 15-hydroxyeicosatetraenoic acid (15-HETE). Although mammalian lipoxygenases are cytoplasmic enzymes, P. aeruginosa LoxA activity is secreted. Taken together, these results suggest a mechanism by which a pathogen-secreted lipoxygenase may modulate host defense and inflammation via alteration of the biosynthesis of local chemical mediators.
机译：在哺乳动物中，脂氧合酶通过引发花生四烯酸转化为有效的生物活性脂质介质（如白三烯和脂蛋白）而在炎症中起关键作用。通常，大多数细菌被认为缺乏脂氧化酶及其多不饱和脂肪酸底物。因此，令人感兴趣的是，通过分析机会细菌病原体铜绿假单胞菌的全基因组序列，发现了与真核脂肪氧化酶具有高度同源性的ORF（PA1169）。使用TLC和液相色谱-UV串联质谱（LC-UV-MS-MS），我们证明PA1169编码细菌脂氧合酶（LoxA），该酶将花生四烯酸转化为15-羟基二十碳四烯酸（15-HETE）。尽管哺乳动物脂加氧酶是细胞质酶，但铜绿假单胞菌LoxA活性却被分泌。综上所述，这些结果表明病原体分泌的脂氧合酶可通过改变局部化学介质的生物合成来调节宿主防御和炎症的机制。

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