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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >PERIOD2::LUCIFERASE real-time reporting of circadian dynamics reveals persistent circadian oscillations in mouse peripheral tissues.
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PERIOD2::LUCIFERASE real-time reporting of circadian dynamics reveals persistent circadian oscillations in mouse peripheral tissues.

机译：PERIOD2 :: LUCIFERASE昼夜节律动态的实时报告揭示了小鼠外周组织中持续的昼夜节律振荡。

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摘要

Mammalian circadian rhythms are regulated by the suprachiasmatic nucleus (SCN), and current dogma holds that the SCN is required for the expression of circadian rhythms in peripheral tissues. Using a PERIOD2::LUCIFERASE fusion protein as a real-time reporter of circadian dynamics in mice, we report that, contrary to previous work, peripheral tissues are capable of self-sustained circadian oscillations for >20 cycles in isolation. In addition, peripheral organs expressed tissue-specific differences in circadian period and phase. Surprisingly, lesions of the SCN in mPer2(Luciferase) knockin mice did not abolish circadian rhythms in peripheral tissues, but instead caused phase desynchrony among the tissues of individual animals and from animal to animal. These results demonstrate that peripheral tissues express self-sustained, rather than damped, circadian oscillations and suggest the existence of organ-specific synchronizers of circadian rhythms at the cell and tissue level.
机译：哺乳动物昼夜节律受上睑交叉神经核（SCN）的调节，目前的教条认为，SCN是外周组织中昼夜节律表达所必需的。使用PERIOD2 :: LUCIFERASE融合蛋白作为小鼠昼夜节律动态的实时报告者，我们报道，与以前的工作相反，外围组织能够自我维持昼夜节律振荡，持续> 20个周期。此外，外周器官在昼夜节律时期和阶段表达组织特异性差异。出人意料的是，mPer2（荧光素酶）敲入小鼠中SCN的损伤并未消除外周组织中的昼夜节律，而是引起了个体动物之间以及动物之间的相位失调。这些结果表明，外周组织表达自我维持而不是衰减的昼夜节律振荡，并暗示在细胞和组织水平上存在昼夜节律的器官特异性同步子。

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