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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Transcriptional termination by RNA polymerase Ⅰ requires the small subunit Rpa12p
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Transcriptional termination by RNA polymerase Ⅰ requires the small subunit Rpa12p

机译：RNA聚合酶Ⅰ终止转录需要Rpa12p小亚基

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摘要

We identify Rpa12p of RNA polymerase Ⅰ (Pol Ⅰ) as a termination factor. Combined analyses using transcription run-on, electron microscopy-visualized chromatin spreading and RT-PCR have been applied to the rRNA-encoding genes of Saccharomyces cerevisiae. These confirm that Pol Ⅰ termination occurs close to the Reb1p-dependent terminator in wild-type strains. However, deletion mutants for the 3′ end-processing enzyme Rnt1p or the Rpa12p subunit of Pol Ⅰ both show Pol Ⅰ transcription in the spacer. For Δrpa12, these spacer polymerases are devoid of nascent transcripts, suggesting they are immediately degraded. The homology of Rpa12p to the small subunit Rpb9p of Pol Ⅱ and Rpc11p of Pol Ⅲ, both implicated in transcriptional termination, points to a common termination mechanism for all three classes of RNA polymerase.
机译：我们确定RNA聚合酶Ⅰ（PolⅠ）的Rpa12p为终止因子。使用转录运行，电子显微镜可视化的染色质铺展和RT-PCR的组合分析已应用于啤酒酵母的rRNA编码基因。这些证实在野生型菌株中PolⅠ终止接近Reb1p依赖性终止子。然而，PolⅠ的3'末端加工酶Rnt1p或Rpa12p亚基的缺失突变体在间隔区均显示PolⅠ的转录。对于Δrpa12，这些间隔聚合酶不含新生的转录本，表明它们会立即降解。 Rpa12p与PolⅡ的小亚基Rpb9p和PolⅢ的Rpc11p的同源性，均与转录终止有关，指出了这三类RNA聚合酶的共同终止机制。

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