...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The minimal promoter plays a major role in silencing of the galago gamma-globin gene in adult erythropoiesis.
【24h】

The minimal promoter plays a major role in silencing of the galago gamma-globin gene in adult erythropoiesis.

机译：最小启动子在成年红细胞生成中加拉戈γ-珠蛋白基因的沉默中起主要作用。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The human gamma-globin gene and its orthologous galago gamma-globin gene evolved from an ancestral epsilon-globin gene. In galago, expression of the gamma-gene remained restricted to the embryonic stage of development, whereas in humans, expression of the gamma-gene was recruited to the fetal stage. To localize the cis-elements responsible for this developmentally distinct regulation, we studied the expression patterns of the human gamma-gene driven by either the human or the galago gamma-promoters in transgenic mice. gamma-gene transcription driven by either promoter reached similar levels in embryonic erythropoiesis. In adult erythropoiesis the gamma-gene was silenced when controlled by the galago gamma-promoter, but it was expressed at a high level when it was linked to the human gamma-promoter. By a series of gamma-promoter truncations the sequences required for the down-regulation of the galago gamma-globin gene were localized to the minimal promoter. Furthermore, by interchanging the TATA, CCAAT,and CACCC elements between the human and galago minimal promoters we found that whereas each box made a developmentally distinctive contribution to gamma-globin gene expression, the CACCC box was largely responsible for the down-regulation of the gamma-gene in adult erythropoiesis.
机译：人γ-珠蛋白基因及其直系同源galagoγ-珠蛋白基因是从祖先ε-珠蛋白基因进化而来的。在加拉戈，伽马基因的表达仍然局限于胚胎的发育阶段，而在人类中，伽马基因的表达被募集到胎儿阶段。为了定位负责这种发育上不同的调控的顺式元件，我们研究了由人类或galagoγ-启动子驱动的人类γ基因在转基因小鼠中的表达模式。由任一启动子驱动的γ基因转录在胚胎红细胞生成中达到相似的水平。在成年的红细胞生成中，当由加拉戈伽玛启动子控制时，伽马基因被沉默，但是当它与人类伽玛启动子连接时，其高水平表达。通过一系列的γ-启动子截短，将加拉戈γ-珠蛋白基因的下调所需的序列定位于最小启动子。此外，通过在人类和加拉哥的最小启动子之间交换TATA，CCAAT和CACCC元件，我们发现，尽管每个框对γ-珠蛋白基因表达都具有发展上的独特贡献，但CACCC框在很大程度上负责了成人红细胞生成中的γ基因。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号