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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Human and yeast Rad52 proteins promote DNA strand exchange.
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Human and yeast Rad52 proteins promote DNA strand exchange.

机译：人和酵母Rad52蛋白促进DNA链交换。

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摘要

Studies of rad52 mutants in Saccharomyces cerevisiae have revealed a critical role of Rad52 protein in double-strand break repair and meiosis, and roles in both RAD51-dependent and -independent pathways of recombination. In vitro, both yeast and human Rad52 proteins play auxiliary roles with RPA in the action of Rad51. Rad52 also has annealing activity and promotes the formation of D-loops in superhelical DNA. The experiments described here show that Homo sapiens (Hs)Rad52 and yeast Rad52 proteins promote strand exchange as well. Strand exchange was promoted by the N-terminal domain of HsRad52 that contains residues 1-237, which includes the residues required to form rings of Rad52, whereas other truncated domains, both N-terminal and C-terminal, were inactive. For both yeast Rad52 and HsRad52, the yield of strand-exchange reactions was proportional to the fractional A.T content of the DNA substrates, but both enzymes catalyzed exchange with substrates that contained up to at least 50% G.C. Observations made on S. cerevisiae Rad52 protein from mutants with severe recombination deficiencies indicate that the strand-exchange activity measured in vitro reflects a biologically significant property of Rad52 protein.
机译：对酿酒酵母中rad52突变体的研究表明，Rad52蛋白在双链断裂修复和减数分裂中起关键作用，并在RAD51依赖性和非依赖性重组途径中发挥作用。在体外，酵母和人类Rad52蛋白均在Rad51的作用中与RPA发挥辅助作用。 Rad52还具有退火活性，并促进超螺旋DNA中D环的形成。此处描述的实验表明，智人（Rs）人Rad52和酵母Rad52蛋白也促进链交换。 HsRad52的N末端结构域促进了链交换，该结构域包含残基1-237，其中包括形成Rad52环所需的残基，而其他截短的结构域（N末端和C末端）均无活性。对于酵母Rad52和HsRad52而言，链交换反应的产率均与DNA底物的A.T含量分数成正比，但两种酶都催化与含有至少50％G.C的底物交换。对来自具有严重重组缺陷的突变体的酿酒酵母Rad52蛋白的观察表明，体外测得的链交换活性反映了Rad52蛋白的生物学显着特性。

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