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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Yeast telomerase RNA: a flexible scaffold for protein subunits.
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Yeast telomerase RNA: a flexible scaffold for protein subunits.

机译：酵母端粒酶RNA：用于蛋白质亚基的柔性支架。

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摘要

In the yeast Saccharomyces cerevisiae, distinct regions of the 1.2-kb telomerase RNA (TLC1) bind to the catalytic subunit Est2p and to accessory proteins. In particular, a bulged stem structure binds the essential regulatory subunit Est1p. We now show that the Est1p-binding domain of the RNA can be moved to three distant locations with retention of telomerase function in vivo. We present the Est1p relocation experiment in the context of a working model for the secondary structure of the entire TLC1 RNA, based on thermodynamic considerations and comparative analysis of sequences from four species. The model for TLC1 has three long quasihelical arms that bind the Ku, Est1p, and Sm proteins. These arms emanate from a central catalytic core that contains the template and Est2p-binding region. Deletion mutagenesis provides evidence that the Sm arm exists in vivo and can be shortened by 42 predicted base pairs with retention of function; therefore, precise positioning of Sm proteins, like Est1p, is not required within telomerase. In the best-studied ribonucleoprotein enzyme, the ribosome, the RNAs have specific three-dimensional structures that orient the functional elements. In the case of yeast telomerase, we propose that the RNA serves a very different function, providing a flexible tether for the protein subunits.
机译：在酵母酿酒酵母中，1.2 kb端粒酶RNA（TLC1）的不同区域与催化亚基Est2p和辅助蛋白结合。尤其是，凸起的茎结构结合了必需的调节亚基Est1p。现在，我们显示RNA的Est1p结合结构域可以在体内保留端粒酶功能的三个位置移动。我们基于热力学考虑和对来自四个物种的序列的比较分析，在整个TLC1 RNA二级结构的工作模型的背景下，介绍了Est1p重定位实验。 TLC1的模型具有三个结合了Ku，Est1p和Sm蛋白的长准螺旋臂。这些臂来自包含模板和Est2p结合区的中央催化核心。缺失诱变提供了证据，表明Sm臂存在于体内，可以被42个预测的碱基对缩短并保留其功能。因此，端粒酶内无需精确定位Sm蛋白（如Est1p）。在研究最深入的核糖核糖核酸酶（核糖体）中，RNA具有定向功能元件的特定三维结构。对于酵母端粒酶，我们建议RNA发挥非常不同的功能，为蛋白质亚基提供灵活的系链。

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