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RNA dynamics in live Escherichia coli cells.

机译:活大肠杆菌细胞中的RNA动态。

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摘要

We describe a method for tracking RNA molecules in Escherichia coli that is sensitive to single copies of mRNA, and, using the method, we find that individual molecules can be followed for many hours in living cells. We observe distinct characteristic dynamics of RNA molecules, all consistent with the known life history of RNA in prokaryotes: localized motion consistent with the Brownian motion of an RNA polymer tethered to its template DNA, free diffusion, and a few examples of polymer chain dynamics that appear to be a combination of chain fluctuation and chain elongation attributable to RNA transcription. We also quantify some of the dynamics, such as width of the displacement distribution, diffusion coefficient, chain elongation rate, and distribution of molecule numbers, and compare them with known biophysical parameters of the E. coli system.
机译:我们描述了一种对大肠杆菌中的RNA分子进行追踪的方法,该方法对mRNA的单拷贝敏感,并且使用该方法,我们发现单个分子可以在活细胞中追踪多个小时。我们观察到RNA分子的独特特征动力学,这些动力学均与原核生物中RNA的已知生命史一致:局部运动与拴接到其模板DNA的RNA聚合物的布朗运动,自由扩散以及聚合物链动力学的一些例子相符似乎是归因于RNA转录的链波动和链伸长的组合。我们还量化了一些动力学,例如位移分布的宽度,扩散系数,链伸长率和分子数目的分布,并将它们与大肠杆菌系统的已知生物物理参数进行比较。

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