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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Genes encoding candidate pheromone receptors in a moth (Heliothis virescens).
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Genes encoding candidate pheromone receptors in a moth (Heliothis virescens).

机译：蛾中编码候选信息素受体的基因（Heliothis virescens）。

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摘要

The remarkable responsiveness of male moths to female released pheromones is based on the extremely sensitive and selective reaction of highly specialized sensory cells in the male antennae. These cells are supposed to be equipped with male-specific receptors for pheromonal compounds, however, the nature of these receptors is still elusive. By using a combination of genomic sequence analysis and cDNA-library screening, we have cloned various cDNAs of the tobacco budworm Heliothis virescens encoding candidate olfactory receptors. A comparison of all identified receptor types not only highlighted their overall high degree of sequence diversity but also led to the identification of a small group of receptors sharing >40% identity. In RT-PCR analysis it was found that distinct members of this group were expressed exclusively in the antennae of male moths. In situ hybridization experiments revealed that the male-specific expression of these receptor types was confined to antennal cells located beneath sensillar hair structures (sensilla triochoidea), which have been shown to contain pheromone-sensitive neurons. Moreover, two-color double in situ-hybridization approaches uncovered that cells expressing one of these receptor types were surrounded by cells expressing pheromone-binding proteins, as expected for a pheromone-sensitive sensillum. These findings suggest that receptors like Heliothis receptor 14-16 (HR14-HR16) may render antennal cells responsive to pheromones.
机译：雄蛾对雌性释放的信息素的显着响应是基于雄性触角中高度专门化的感觉细胞的极其敏感和选择性的反应。假定这些细胞配备有雄性特异性信息素化合物受体，但是，这些受体的性质仍然难以捉摸。通过结合使用基因组序列分析和cDNA文库筛选，我们已经克隆了烟草芽虫Heliothis virescens的各种cDNA，它们编码候选嗅觉受体。所有已鉴定受体类型的比较不仅突出了它们总体上高度的序列多样性，而且还导致鉴定出一小群具有> 40％同一性的受体。在RT-PCR分析中，发现该组的不同成员仅在雄蛾的触角中表达。原位杂交实验表明，这些受体类型的雄性特异性表达仅限于触角毛发结构（触角毛线虫）下方的触角细胞，该触角细胞已显示含有信息素敏感的神经元。此外，双色双原位杂交方法发现，表达这些受体类型之一的细胞被表达信息素结合蛋白的细胞所包围，正如对信息素敏感的感觉分子所期望的那样。这些发现表明，像Heliothis受体14-16（HR14-HR16）这样的受体可能使触角细胞对信息素产生反应。

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