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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Rhodopsin kinase activity modulates the amplitude of the visual response in Drosophila.
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Rhodopsin kinase activity modulates the amplitude of the visual response in Drosophila.

机译：视紫红质激酶活性调节果蝇视觉反应的幅度。

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摘要

A feature shared between Drosophila rhodopsin and nearly all other G protein-coupled receptors is agonist-dependent protein phosphorylation. Despite extensive analyses of Drosophila phototransduction, the identity and function of the rhodopsin kinase (RK) have been elusive. Here, we provide evidence that G protein-coupled receptor kinase 1 (GPRK1), which is most similar to the beta-adrenergic receptor kinases, G protein-coupled receptor kinase 2 (GRK2) and GRK3, is the fly RK. We show that GPRK1 is enriched in photoreceptor cells, associates with the major Drosophila rhodopsin, Rh1, and phosphorylates the receptor. As is the case with mammalian GRK2 and GRK3, Drosophila GPRK1 includes a C-terminal pleckstrin homology domain, which binds to phosphoinositides and the Gbetagamma subunit. To address the role of GPRK1, we generated transgenic flies that expressed higher and lower levels of RK activity. Those flies with depressed levels of RK activity displayed a light response with a much larger amplitude than WT. Conversely, the amplitude of the light response was greatly suppressed in transgenic flies expressing abnormally high levels of RK activity. These data point to an evolutionarily conserved role for GPRK1 in modulating the amplitude of the visual response.
机译：果蝇视紫红质和几乎所有其他G蛋白偶联受体之间共有的特征是激动剂依赖性蛋白磷酸化。尽管对果蝇光转导进行了广泛的分析，但视紫红质激酶（RK）的身份和功能却难以捉摸。在这里，我们提供的证据表明，与β-肾上腺素受体激酶，G蛋白偶联受体激酶2（GRK2）和GRK3最相似的G蛋白偶联受体激酶1（GPRK1）是RK。我们显示GPRK1富含光感受器细胞，与主要果蝇视紫红质Rh1缔合，并使受体磷酸化。与哺乳动物GRK2和GRK3一样，果蝇GPRK1包含一个C端pleckstrin同源域，该域与磷酸肌醇和Gbetagamma亚基结合。为了解决GPRK1的作用，我们生成了表达更高和更低水平RK活性的转基因果蝇。那些RK活性降低的果蝇表现出比WT大得多的光响应。相反，在表达异常高水平的RK活性的转基因果蝇中，光响应的幅度被大大抑制。这些数据表明GPRK1在调节视觉响应幅度方面在进化上保守。

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