Triggered amplification by hybridization chain reaction

Dirks RM; Pierce NA

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Triggered amplification by hybridization chain reaction

机译：通过杂交链反应触发扩增

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摘要

We introduce the concept of hybridization chain reaction (HCR), in which stable DNA monomers assemble only upon exposure to a target DNA fragment. In the simplest version of this process, two stable species of DNA hairpins coexist in solution until the introduction of initiator strands triggers a cascade of hybridization events that yields nicked double helices analogous to alternating copolymers. The average molecular weight of the HCR products varies inversely with initiator concentration. Amplification of more diverse recognition events can be achieved by coupling HCR to aptamer triggers. This functionality allows DNA to act as an amplifying transducer for biosensing applications.

机译：我们介绍了杂交链反应（HCR）的概念，其中稳定的DNA单体仅在暴露于目标DNA片段时才组装。在此过程的最简单形式中，溶液中共存在两种稳定的DNA发夹物种，直到引发剂链的引入触发了一系列级联的杂交事件，产生了类似于交替共聚物的带切口双螺旋。 HCR产品的平均分子量与引发剂浓度成反比。通过将HCR与适体触发物偶联，可以实现更多种多样的识别事件。此功能使DNA可以用作生物传感应用中的放大换能器。

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《Proceedings of the National Academy of Sciences of the United States of America》 |2004年第43期|p.15275-15278|共4页
作者
Dirks RM; Pierce NA;
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作者单位

Department of Chemistry, California Institute of Technology, Pasadena, CA 91125, USA.;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
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机译：基于目标触发的杂交链反应介导的DNA酶和Pt纳米链原位生成的无标记级联扩增策略，用于凝血酶的灵敏视觉检测
2. Label-free and amplified electrogenerated chemiluminescence biosensing for the detection of thymine DNA glycosylase activity using DNA-functionalized gold nanoparticles triggered hybridization chain reaction [J] . Bai Wanqiao, Wei Yingying, Zhang Yuecheng, Analytica chimica acta . 2019,第期

机译：使用DNA官能化金纳米粒子触发杂交链反应的标记和扩增的胸腺嘧啶DNA糖基酶活性的无标记和扩增的电化学化学发光生物溶解
3. Dandelion-like CuO microspheres decorated with Au nanoparticle modified biosensor for Hg2+ detection using a T-Hg2+-T triggered hybridization chain reaction amplification strategy [J] . Yu Yujie, Yu Chao, Gao Rufei, Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices . 2019,第期

机译：蒲公英的CuO微球，用Au纳米粒子改性的生物传感器，用于HG2 +检测使用T-HG2 + -T触发杂交链反应扩增策略
4. Amplification and modulation of fluorescent signals by using hybridization chain reactions for multiplexed sensing of biomolecules in a one-pot [C] . Takahiro Nishimura, Yusuke Ogura, Kenji Ymada, Nanoscale imaging, sensing, and actuation for biomedical applications XI . 2014

机译：通过杂交链反应对一锅中生物分子进行多重传感的荧光信号放大和调制
5. Multifunctional Nanomaterials Utilizing Hybridization Chain Reaction for Molecular Diagnostics and Bioanalytical Applications. [D] . Rana, Md. Muhit. 2017

机译：利用杂交链反应的多功能纳米材料用于分子诊断和生物分析应用。
6. From the Cover: Triggered amplification by hybridization chain reaction [O] . Robert M. Dirks, Niles A. Pierce 2004

机译：从封面：通过杂交链反应引发的扩增
7. Triggered amplification by hybridization chain reaction [O] . Dirks Robert M., Pierce Niles A. 2004

机译：通过杂交链反应触发扩增
8. Population Genetics Study of Anopheles Darlingi (Diptera: Culicidae) from Colombia Based on Random Amplified Polymorphic DNA-Polymerase Chain Reaction and Amplified Fragment Length Polymorphism Markers [R] . Gonzalez, R., Wilkerson, R., Suarez, M. F., 2007

机译：基于随机扩增多态性DNa聚合酶链反应和扩增片段长度多态性标记的哥伦比亚按蚊Darleri（双翅目：蚊科）的群体遗传学研究

Triggered amplification by hybridization chain reaction

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