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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >A rare genotype of Cryptococcus gattii caused the cryptococcosis outbreak on Vancouver Island (British Columbia, Canada).
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A rare genotype of Cryptococcus gattii caused the cryptococcosis outbreak on Vancouver Island (British Columbia, Canada).

机译：加蒂隐球菌的一种罕见基因型导致温哥华岛（加拿大不列颠哥伦比亚省）爆发隐球菌病。

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摘要

Cryptococcus gattii causes life-threatening infection of the pulmonary and central nervous systems in hosts with normal immunity and traditionally has been considered to be restricted geographically to tropical and subtropical climates. The recent outbreak of C. gattii in the temperate climate of Vancouver Island, BC, Canada, led to a collaborative investigation. The objectives of the current study were to ascertain the environmental source of the outbreak infections, survey the molecular types of the outbreak and environmental cryptococcal isolates, and determine the extent of genetic diversity among the isolates. PCR-fingerprinting and amplified fragment length polymorphism (AFLP) were used to examine the genotypes, and mating assays were performed to determine the mating type of the isolates. All outbreak and environmental isolates belonged to C. gattii. Concordant results were obtained by using PCR-fingerprinting and AFLP analysis. The vast majority of clinical and veterinary infections were causedby isolates of the molecular type VGII/AFLP6, but two were caused by molecular type VGI/AFLP4. All environmental isolates belonged to molecular type VGII/AFLP6. Two or three subtypes were observed within VGII/AFLP6 among outbreak and environmental isolates. All mating-competent isolates were of the alpha-mating type. The emergence of this usually tropical pathogen on Vancouver Island highlights the changing distribution of this genotype and emphasizes the importance of an ongoing collaborative effort to monitor the global epidemiology of this yeast.
机译：加蒂隐球菌在免疫力正常的宿主中导致威胁生命的肺和中枢神经系统感染，传统上被认为在地理上仅限于热带和亚热带气候。最近在加拿大不列颠哥伦比亚省温哥华岛的温带气候下爆发的加迪梭菌导致了合作调查。当前研究的目的是确定爆发感染的环境来源，调查爆发和环境隐球菌分离株的分子类型，并确定分离株之间的遗传多样性程度。 PCR指纹图谱和扩增的片段长度多态性（AFLP）用于检查基因型，并进行交配测定以确定分离株的交配类型。所有暴发和环境隔离株均属于加迪梭菌。通过使用PCR指纹图谱和AFLP分析获得一致的结果。绝大部分的临床和兽医感染是由分子类型为VGII / AFLP6的分离株引起的，但其中两个是由分子类型VGI / AFLP4引起的。所有环境分离物均属于分子类型VGII / AFLP6。在VGII / AFLP6病毒和环境分离株中观察到2或3个亚型。所有具有交配能力的分离株均为α-交配型。这种通常是热带病原体在温哥华岛上的出现突出了该基因型的分布变化，并强调了正在进行的合作努力以监测这种酵母的全球流行病学的重要性。

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